Genotoxic effect of 2,2'-bis(bicyclo[2.2.1] heptane) on bacterial cells
| Autor: | V. O. Samoilov, S. V. Shorunov, A. Kessenikh, A. L. Maksimov, E. Gnuchikh, Ilya V. Manukhov, Lev S. Yaguzhinsky, Sergey V. Bazhenov, Maxim V. Bermeshev, V G Pevgov |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Luminescence Alkylation Biosensing Techniques medicine.disease_cause 01 natural sciences Biochemistry Physical Chemistry chemistry.chemical_compound Superoxides SOS response chemistry.chemical_classification Multidisciplinary Superoxide Physics Electromagnetic Radiation Chemical Reactions Detectors Oxides Peroxides Nucleic acids Chemistry Physical Sciences Engineering and Technology Medicine Research Article Anions Stereochemistry DNA damage Science Equipment Oxidative phosphorylation 010402 general chemistry Regulon 03 medical and health sciences Bridged Bicyclo Compounds Oxidation medicine Genetics Escherichia coli Luciferase Ions Reactive oxygen species Biology and life sciences Dose-Response Relationship Drug Chemical Compounds Correction DNA Hydrogen Peroxide Hydrocarbons 0104 chemical sciences Oxidative Stress 030104 developmental biology Biosensors chemistry bacteria Genotoxicity DNA Damage Mutagens |
| Zdroj: | PLoS ONE, Vol 15, Iss 8, p e0228525 (2020) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The toxic effect of strained hydrocarbon 2,2'-bis (bicyclo[2.2.1]heptane) (BBH) was studied using whole-cell bacterial lux-biosensors based on Escherichia coli cells in which luciferase genes are transcriptionally fused with stress-inducible promoters. It was shown that BBH has the genotoxic effect causing bacterial SOS response however no alkylating effect has been revealed. In addition to DNA damage, there is an oxidative effect causing the response of OxyR/S and SoxR/S regulons. The most sensitive to BBH lux-biosensor was E. coli pSoxS-lux which reacts to the appearance of superoxide anion radicals in the cell. It is assumed that the oxidation of BBH leads to the generation of reactive oxygen species, which provide the main contribution to the genotoxicity of this substance. |
| Databáze: | OpenAIRE |
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