A simple layer-stacking technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels
| Autor: | Yuting Niu, Somayeh Zanganeh, Héloïse Mary, Jeremy J. Mao, Shu-Kai Hu, Ali Khademhosseini, Goro Choi, Hyojin Ko, Samad Ahadian, Yunzhi Yang, Kasinan Suthiwanich, Kirsten Fetah |
|---|---|
| Přispěvatelé: | Center for Minimally Invasive Therapeutics [Los Angeles] (C-MIT), University of California [Los Angeles] (UCLA), University of California-University of California, Henry Samueli School of Engineering and Applied Sciences [Los Angeles], Tokyo Institute of Technology [Tokyo] (TITECH), Pôle Odontologie [CHU Reims], Centre Hospitalier Universitaire de Reims (CHU Reims), Plateforme technologique Biomatériaux et Microfluidique - Biomaterials and Microfluidics technologic Platform, Institut Pasteur [Paris], National Taiwan University [Taiwan] (NTU), Stanford University, Center for Craniofacial Regeneration [New York], Columbia University [New York], University of California (UC)-University of California (UC), Institut Pasteur [Paris] (IP) |
| Rok vydání: | 2019 |
| Předmět: |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Light Polymers Swine 02 engineering and technology Biochemistry Gelatin Mice chemistry.chemical_compound Tissue engineering Fluorescence microscope MESH: Animals MESH: Swine [SPI.FLUID]Engineering Sciences [physics]/Reactive fluid environment Hydrogels Serum Albumin Bovine General Medicine 021001 nanoscience & nanotechnology MESH: Methacrylates MESH: Polymers Cross-Linking Reagents Self-healing hydrogels Methacrylates 0210 nano-technology Layer (electronics) Biotechnology MESH: Hydrogels Materials science food.ingredient Ultraviolet Rays MESH: Cross-Linking Reagents 0206 medical engineering Biomedical Engineering Stacking Bioengineering macromolecular substances complex mixtures Article Biomaterials food MESH: Mechanical Phenomena Animals Humans MESH: Mice Mechanical Phenomena MESH: Humans Layer by layer technology industry and agriculture 020601 biomedical engineering MESH: Light Chemical engineering chemistry NIH 3T3 Cells MESH: Ultraviolet Rays Ethylene glycol MESH: Serum Albumin Bovine MESH: NIH 3T3 Cells |
| Zdroj: | Biofabrication Biofabrication, IOP Publishing, 2019, 11 (2), pp.025014. ⟨10.1088/1758-5090/ab08b5⟩ Biofabrication, 2019, 11 (2), pp.025014. ⟨10.1088/1758-5090/ab08b5⟩ |
| ISSN: | 1758-5090 1758-5082 |
| Popis: | International audience; Physicochemical and biological gradients are desirable features for hydrogels to enhance their relevance to biological environments for three-dimensional (3D) cell culture. Therefore, simple and efficient techniques to generate chemical, physical and biological gradients within hydrogels are highly desirable. This work demonstrates a technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels by stacking and crosslinking prehydrogel solution in a layer by layer manner. Partial crosslinking of the hydrogel allows mixing of prehydrogel solution with the previous hydrogel layer, which makes a smooth gradient profile, rather than discrete layers. This technique enables the generation of concentration gradients of bovine serum albumin in both gelatin methacryloyl (GelMA) and poly(ethylene glycol) diacrylate hydrogels, as well as mechanical gradients across a hydrogel containing varying gel concentrations. Fluorescence microscopy, mechanical testing, and scanning electron microscopy show that the gradient profiles can be controlled by changing both the volume and concentration of each layer as well as intensity of UV exposure. GelMA hydrogel gradients with different Young's moduli were successfully used to culture human fibroblasts. The fibroblasts migrated along the gradient axis and showed different morphologies. In general, the proposed technique provides a rapid and simple approach to design and fabricate 3D hydrogel gradients for in vitro biological studies and potentially for in vivo tissue engineering applications. |
| Databáze: | OpenAIRE |
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