The antidepressant-like effect of Hypericum caprifoliatum Cham & Schlecht (Guttiferae) on forced swimming test results from an inhibition of neuronal monoamine uptake
| Autor: | Stela Maris Kuze Rates, Alexandre de Barros Falcão Ferraz, Jean-Claude do Rego, Alice Fialho Viana, Jean Costentin, Ana Paula Machado Heckler, Gilsane Lino von Poser |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Serotonin Apomorphine Dopamine Phloroglucinol Hypothermia In Vitro Techniques Motor Activity Pharmacology Binding Competitive Mice Norepinephrine Cellular and Molecular Neuroscience chemistry.chemical_compound Monoaminergic medicine Animals Neurotransmitter Uptake Inhibitors Rats Wistar Swimming SCH-23390 Dose-Response Relationship Drug Depression Plant Extracts Antidepressive Agents Rats Monoamine neurotransmitter chemistry Dopamine Agonists medicine.symptom Sulpiride Hypericum Synaptosomes Behavioural despair test medicine.drug |
| Zdroj: | Neuropharmacology. 49:1042-1052 |
| ISSN: | 0028-3908 |
| Popis: | A crude (ECH) and a purified cyclohexane extract (HCP) of Hypericum caprifoliatum and their main phloroglucinol derivative (HC1) were evaluated regarding their action on monoaminergic systems, more precisely on dopamine. In rats and mice forced swimming test, ECH and HCP dose-dependently reduced the immobility time. The effect of the highest dose was prevented by a prior administration of either sulpiride or SCH 23390 (D 2 and D 1 dopamine receptor antagonist, respectively). HCP (360 mg/kg) decreased the locomotor activity of mice. ECH (90 mg/kg) caused hypothermia and potentiated apomorphine-induced (16 mg/kg) hypothermia in mice. HCP and HC1 inhibited, in a concentration-dependent and monophasic manner, the [ 3 H]-DA, [ 3 H]-NA and [ 3 H]-5HT synaptosomal uptakes, but did not prevent the binding of specific ligands to the monoamine transporters. Moreover, when tested at the concentrations corresponding to its IC 50 on [ 3 H]-DA uptake, HC1 did not induce a significant [ 3 H]-DA release, while at a higher concentration (200 ng/ml) it enhanced significantly (by 12%) the synaptosomal DA release. These data suggest that the antidepressant-like effect of H. caprifoliatum on the forced swimming test is due to an increase in monoaminergic transmission, resulting from monoamine uptake inhibition, more potently of dopamine, which may be related to their phloroglucinol contents. |
| Databáze: | OpenAIRE |
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