Rapid vascularization of tissue-engineered vascular grafts in vivo by endothelial cells in co-culture with smooth muscle cells
| Autor: | Huimin Huang, Yanzhong He, Wei Wang, Zhenyu Wang, Wei Fu, Xindi Yu |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Materials science Angiogenesis Biomedical Engineering Biophysics Mice Nude Neovascularization Physiologic Bioengineering Biomaterials Mice Tissue engineering Smooth muscle In vivo medicine Animals Tissue engineered Decellularization Tissue Engineering Muscle Smooth Coculture Techniques Cell biology medicine.anatomical_structure Blood Vessels Perfusion Biomedical engineering Blood vessel |
| Zdroj: | Journal of Materials Science: Materials in Medicine. 23:1109-1117 |
| ISSN: | 1573-4838 0957-4530 |
| DOI: | 10.1007/s10856-012-4576-8 |
| Popis: | A major challenge facing the development of tissue-engineered vascular grafts (TEVGs), promising living replacements for diseased vascular structures, is enhancing angiogenesis. To promote rapid vascularization, endothelial cells (ECs) were co-cultured with smooth muscle cells (SMCs) in decellularized small intestinal submucosa scaffolds to regenerate angiogenic-TEVGs (A-TEVGs). Observation of the A-TEVGs at 1 month post-implantation revealed that a rich network of neocapillaries lining the blood vessel wall had developed; that the ECs of the neovasculatures had been derived from previously seeded ECs and later invading ECs of the host's vascular bed; that tissue vascularization had not significantly impaired mechanical properties; and that the maximal tensile strength of the A-TEVGs was of the same order of magnitude as that of native porcine femoral arteries. These results indicate that of the co-culturing of ECs with SMCs could enhance vascularization of TEVGs in vivo, possibly increasing graft perfusion and host integration. |
| Databáze: | OpenAIRE |
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