In vitro organogenesis and genetic transformation of mandarin cultivars
| Autor: | Marcelo Favaretto Correa, Ricardo Harakava, Leonardo Soriano, Francisco de Assis Alves Mourão Filho, Beatriz Madalena Januzzi Mendes, Eveline Carla da Rocha Tavano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
antimicrobial peptide
citocinina Organogenesis cultivar improvement Plant Science Genetically modified crops huanglongbing transgenic plants Biology lcsh:Plant culture plantas transgênicas peptídeo antimicrobiano cytokinin chemistry.chemical_compound lcsh:SB1-1110 Cultivar fungi melhoramento de cultivares food and beverages In vitro Transformation (genetics) Horticulture CITOCIMINAS chemistry Shoot Cytokinin Agronomy and Crop Science Food Science Explant culture |
| Zdroj: | Revista Brasileira de Fruticultura, Vol 41, Iss 2 (2019) Revista Brasileira de Fruticultura v.41 n.2 2019 Revista brasileira de fruticultura Sociedade Brasileira de Fruticultura (SBF) instacron:SBFRU Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1806-9967 |
| Popis: | The in vitro organogenesis of Fremont (Citrus clementina x ), Citrus reticulataThomas (Citrus reticulata), and Nules (Citrus clementina) mandarins was evaluated aiming to optimize a regeneration protocol that could be applied in genetic transformation. The use of epicotyl-derived explants resulted in higher explant responsiveness and number of shoots developed per explant when compared with the use of internodal-derived explants. The highest efficiency in shoot regeneration was observed in the presence of 1 mg L-1 of BAP, regardless of the explant type and cultivar. The in vitro organogenesis protocol produced transgenic plants from three mandarin cultivars expressing attA gene under the control of phloem-specific promoters. |
| Databáze: | OpenAIRE |
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