Ligand diffusion enables force‐independent cell adhesion via activating α5β1 integrin and initiating rac and RhoA signaling
| Autor: | Leixiao Yu, Elisabetta Ada Cavalcanti-Adam, Weifeng Zhao, Rainer Haag, Britta Trappmann, Jose Luis Cuellar Camacho, Andrew W. Holle, Qiang Wei, Joachim P. Spatz, Jennifer L. Young, Changsheng Zhao, Matthias F. Melzig, Yong Hou, Wenyan Xie, Chong Cheng |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
RHOA Integrin Cell Intracellular Space 02 engineering and technology Ligands 010402 general chemistry Mechanotransduction Cellular 01 natural sciences Cell Line Diffusion Cell Adhesion medicine Animals Humans General Materials Science Mechanotransduction Cell adhesion biology Ligand Mechanical Engineering 021001 nanoscience & nanotechnology rac GTP-Binding Proteins 0104 chemical sciences medicine.anatomical_structure Mechanics of Materials biology.protein Biophysics Lamellipodium Stem cell rhoA GTP-Binding Protein 0210 nano-technology Integrin alpha5beta1 Signal Transduction |
| Zdroj: | Advanced Materials |
| Popis: | Cells reside in a dynamic microenvironment in which adhesive ligand availability, density, and diffusivity are key factors regulating cellular behavior. Here, the cellular response to integrin-binding ligand dynamics by directly controlling ligand diffusivity via tunable ligand-surface interactions is investigated. Interestingly, cell spread on the surfaces with fast ligand diffusion is independent of myosin-based force generation. Fast ligand diffusion enhances α5β1 but not αvβ3 integrin activation and initiates Rac and RhoA but not ROCK signaling, resulting in lamellipodium-based fast cell spreading. Meanwhile, on surfaces with immobile ligands, αvβ3 and α5β1 integrins synergistically initiate intracellular-force-based canonical mechanotransduction pathways to enhance cell adhesion and osteogenic differentiation of stem cells. These results indicate the presence of heretofore-unrecognized pathways, distinct from canonical actomyosin-driven mechanisms, that are capable of promoting cell adhesion. |
| Databáze: | OpenAIRE |
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