A transcriptome analysis reveals a role for the indole GLS-linked auxin biosynthesis in secondary dormancy in rapeseed (Brassica napus L.)
| Autor: | Xin Yi, XiangXiang Zhao, Jinfang Chu, Tang Tang, Chen Guimin, Wen-qi Fan, Lei Liu, Qiuhuan Guo, FuXia Liu |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Indoles Secondary dormancy Glucosinolates Brassica Secondary Metabolism Germination RNA-Seq Plant Science Biology Genes Plant 01 natural sciences Transcriptome 03 medical and health sciences chemistry.chemical_compound Plant Growth Regulators Phytohormone Auxin lcsh:Botany Secondary metabolism chemistry.chemical_classification Indoleacetic Acids Volunteer plant Gene Expression Profiling Brassica napus food and beverages Plant Dormancy biology.organism_classification lcsh:QK1-989 030104 developmental biology Biochemistry chemistry Glucosinolate Dormancy RNA-seq analysis Metabolic Networks and Pathways Research Article 010606 plant biology & botany |
| Zdroj: | BMC Plant Biology BMC Plant Biology, Vol 19, Iss 1, Pp 1-18 (2019) |
| ISSN: | 1471-2229 |
| Popis: | Background Brassica napus L. has little or no primary dormancy, but exhibits great variation in secondary dormancy. Secondary dormancy potential in oilseed rape can lead to the emergence of volunteer plants that cause genetic contamination, reduced quality and biosafety issues. However, the mechanisms underlying secondary dormancy are poorly understood. In this study, cultivars Huaiyou-WSD-H2 (H) and Huaiyou-SSD-V1 (V), which exhibit low (approximately 5%) and high (approximately 95%) secondary dormancy rate, respectively, were identified. Four samples, before (Hb and Vb) and after (Ha and Va) secondary dormancy induction by polyethylene glycol (PEG), were collected to identify the candidate genes involved in secondary dormancy via comparative transcriptome profile analysis. Results A total of 998 differentially expressed genes (DEGs), which are mainly involved in secondary metabolism, transcriptional regulation, protein modification and signaling pathways, were then detected. Among these DEGs, the expression levels of those involved in the sulfur-rich indole glucosinolate (GLS)-linked auxin biosynthesis pathway were markedly upregulated in the dormant seeds (Va), which were validated by qRT-PCR and subsequently confirmed via detection of altered concentrations of indole-3-acetic acid (IAA), IAA conjugates and precursors. Furthermore, exogenous IAA applications to cultivar H enhanced secondary dormancy. Conclusion This study first (to our knowledge) elucidated that indole GLS-linked auxin biosynthesis is enhanced during secondary dormancy induced by PEG, which provides valuable information concerning secondary dormancy and expands the current understanding of the role of auxin in rapeseed. Electronic supplementary material The online version of this article (10.1186/s12870-019-1866-z) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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