Whole Genome Expression in Peripheral-Blood Samples of Workers Professionally Exposed to Polycyclic Aromatic Hydrocarbons
| Autor: | Wen-Yi Lin, Jie-Len Huang, Tzu-Chi Lee, Chia-Tsuan Huang, Ming-Tsang Wu, Ming-Yen Lin, Chi-Kung Ho, Chiung-Yu Peng, Chih-Hong Pan, Ting-Yu Chou, Hung-Ju Su, Weihsin Wang, I-Chen Wu |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
Urinary system Air Pollutants Occupational Naphthols Urine Biology Real-Time Polymerase Chain Reaction Toxicology Article Andrology chemistry.chemical_compound Occupational Exposure Gene expression Humans Polycyclic Aromatic Hydrocarbons Coke Gene Oligonucleotide Array Sequence Analysis Genetics Regulation of gene expression Creatinine Pyrenes Genome Human Gene Expression Profiling General Medicine Phenanthrenes Gene expression profiling Real-time polymerase chain reaction Gene Expression Regulation chemistry Biomarkers |
| Zdroj: | Chemical Research in Toxicology. 24:1636-1643 |
| ISSN: | 1520-5010 0893-228X |
| DOI: | 10.1021/tx200181q |
| Popis: | This study aims to examine global gene expression profiles before and after the work-shift among coke-oven workers (COW). COW work six consecutive days and then take two days off. Two blood and urine samples in each worker were collected before starting to work after two-days off and end-of-shift in the sixth-day work in 2009. Altered gene expressions (ratio of gene expression levels between end-of-shift and pre-shift work) were performed by Human OneArray expression system which probes ∼30,000-transcription expression profiling of human genes. Sixteen workers, all men, were enrolled in this study. Median urinary 1-hydroxypyrene (1OHP) levels (μmole/mole creatinine) in end-of-shift work were significantly higher than those in pre-shift work (2.58 vs. 0.29, p = 0.0002). Among the 20,341 genes which passed experimental quality control, 26 gene expression changes, 7 positive and 19 negative, were highly correlated with across-the-shift urinary 1OHP levels (end-of-shift – pre-shift 1OHP) (p-value < 0.001). The high and low exposure groups of across-the-shift urinary 1OHP levels dichotomized in ∼2.00 μmole/mole creatinine were able to be distinguished by these 26 genes. Some of them are known to be involved in apoptosis, chromosome stability/DNA repair, cell cycle control/tumor suppressor, cell adhesion, development/spermatogenesis, immune function, and neuronal cell function. These findings in COW will be an ideal model to study the relationship of PAHs exposure with acute changes of gene expressions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|