β-Galactoside-mediated tissue organization during islet reconstitution
| Autor: | Nobuhiko Kojima, Sae Kamitori, Yasuhiro Ozeki |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
endocrine system Sugar chain ECA Erythrina cristagalli agglutinin LCA Lens culinaris agglutinin α-d-mannosyl group β-Galactoside WGA wheat germ agglutinin MAA Maackia amurensis agglutinin Cell Biomedical Engineering ConA concanavalin A DMEM Dulbecco's Modified Eagle's Medium Biomaterials 03 medical and health sciences chemistry.chemical_compound MC methylcellulose Agglutinin medicine Lactose lcsh:QH573-671 Inhibitory effect geography lcsh:R5-920 geography.geographical_feature_category biology lcsh:Cytology Lectin UEA Ulex europaeus agglutinin SSA Sambucus sieboldiana agglutinin RCA Ricinus communis agglutinin Islet Galactoside Cell biology 030104 developmental biology medicine.anatomical_structure chemistry Biochemistry Cell culture Islet-like structure Islet reconstitution biology.protein Original Article FITC fluorescein isothiocyanate lcsh:Medicine (General) Developmental Biology |
| Zdroj: | Regenerative Therapy, Vol 3, Iss C, Pp 11-14 (2016) Regenerative Therapy |
| ISSN: | 2352-3204 |
| DOI: | 10.1016/j.reth.2016.01.006 |
| Popis: | We have previously reported that multi-cellular heteroaggregates comprising murine pancreatic α (αTC1.6) and β (MIN6-m9) cell lines spontaneously acquired islet-like architecture and displayed higher insulin secretion rates. However, the mechanisms of self-organization remain unclear. The objective of this study is to examine the possibility that a sugar chain participates in the mutual recognition of the cells during reconstitution of the islet-like structure in vitro. Using a lectin-binding assay, we identified Erythrina cristagalli agglutinin (ECA), which particularly recognizes the β-galactoside structure on the surfaces of MIN6-m9 cells. The self-organization of αTC1.6 and MIN6-m9 was obstructed using ECA-bound MIN6-m9 cells. Lactose neutralized the ECA's inhibitory effect on the autonomous rearrangement of αTC1.6 and MIN6-m9 cells, indicating that the inhibition of cell arrangement by ECA was mediated via β-galactoside. We concluded that a β-galactoside sugar chain was central to the reconstitution of the pancreatic islet-like architecture in vitro. Highlights • Erythrina cristagalli agglutinin (ECA) lectin bound to the surfaces of MIN6-m9 cells. • ECA-binding had little toxicity on MIN6-m9 cells. • ECA-bound MIN6-m9 cells prevented self-organization of islet-like tissues. |
| Databáze: | OpenAIRE |
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