Enzyme-Treated Zizania latifolia Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation
| Autor: | Young Hee Lim, Joo Myung Moon, Paul Clayton, Mirae An, Hyungkeun Kim, Hyun Soo Ko |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Physiology Photoaging Clinical Biochemistry antiwrinkle Matrix metalloproteinase Biochemistry Cathepsin B Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lysosome medicine Secretion Molecular Biology chemistry.chemical_classification integumentary system lcsh:RM1-950 matrix metalloproteinases Zizania latifolia Cell Biology medicine.disease Molecular biology Hairless lysosomal shedding lcsh:Therapeutics. Pharmacology 030104 developmental biology medicine.anatomical_structure Enzyme chemistry 030220 oncology & carcinogenesis ultraviolet A Tricin sense organs |
| Zdroj: | Antioxidants Volume 9 Issue 10 Antioxidants, Vol 9, Iss 912, p 912 (2020) |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox9100912 |
| Popis: | Ultraviolet A (UVA) is a risk factor for photoaging and wrinkle formation. Zizania latifolia is an herbaceous perennial plant. It contains many bioactive compounds such as tricin that show antioxidative and anti-inflammatory effects. The aim of this study was to investigate the antiwrinkle effect of a mixture of hydrolytic enzyme (cellulase, hemicellulase and pectinase)-treated Z. latifolia extract (ZLE) and tricin on UVA-irradiated human dermal fibroblasts (HDFs) and SKH-1 hairless mice. Treatment of UVA-irradiated HDF cells with ZLE and tricin significantly decreased UVA induced-plasma membrane rupture, generation of ROS, expression levels of total and secreted lysosomal associated membrane protein (LAMP-1), cathepsin B and metalloproteinases (MMPs) and inhibited NF-&kappa B activation. In the animal study, UVA-damaged epidermal and dermal tissues were repaired by the ZLE and tricin treatments. Administration of ZLE or tricin to UVA-irradiated animals recovered skin surface moisture and collagen fiber in dermal tissue. Treatment of ZLE or tricin decreased wrinkle formation, secretion of MMPs and expression levels of vascular endothelial growth factor (VEGF) and cathepsin B, and increased the expression level of collagen-1 in UVA-irradiated animals. Overall, the ZLE and tricin treatments decreased the skin damage induced by UVA irradiation via inhibition of lysosomal exocytosis and ROS generation. Therefore, ZLE and tricin are promising as antiwrinkle and antiphotoaging agents. |
| Databáze: | OpenAIRE |
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