A structurally novel staphylococcal protein A from the V8 strain
| Autor: | Gilles Prévost, Y. Piemont, I. Mazurier, V Finck-Barbançon |
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| Rok vydání: | 1992 |
| Předmět: |
Reading Frames
Staphylococcus aureus Sequence analysis Molecular Sequence Data Molecular Conformation Biology medicine.disease_cause Microbiology chemistry.chemical_compound Sequence Homology Nucleic Acid Escherichia coli Genetics medicine Amino Acid Sequence Cloning Molecular Codon Staphylococcal Protein A Molecular Biology chemistry.chemical_classification Methionine Base Sequence Binding protein Nucleic acid sequence Molecular biology Amino acid chemistry Biochemistry Mutagenesis Site-Directed biology.protein Leucine Protein A |
| Zdroj: | FEMS Microbiology Letters. 91:1-8 |
| ISSN: | 0378-1097 |
| DOI: | 10.1016/0378-1097(92)90554-2 |
| Popis: | Protein A from the Staphylococcus aureus strain V8 has a molecular mass about 8000 Da less than that of known proteins A. The corresponding gene was cloned and expressed in Escherichia coli. Sequence analysis of this structurally new protein A revealed that it lacked an IgG-binding domain (58 amino acids), and that it also lacked two octapeptide repetitions located in the membrane/wall attaching region. Contrary to what had been proposed previously, the first translated amino acid is probably not a leucine, but very likely a methionine located 12 residues upstream. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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