Importance of total and measured free testosterone in diagnosis of male hypogonadism: immunoassay versus mass spectrometry in a population of healthy young/middle-aged blood donors
| Autor: | A Del Grosso, P Agretti, Caterina Pelosini, M. R. Sessa, D Canale, Alessandro Saba, L. Bianchi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Percentile medicine.medical_specialty Endocrinology Diabetes and Metabolism Population Urology Blood Donors 030209 endocrinology & metabolism Reference range testosterone hypogonadism Immunoassay mass spectrometry men healthy donors men healthy donors 03 medical and health sciences 0302 clinical medicine Endocrinology Sex hormone-binding globulin Reference Values Tandem Mass Spectrometry Humans hypogonadism Medicine Cutoff education mass spectrometry Immunoassay education.field_of_study biology Free testosterone medicine.diagnostic_test business.industry Testosterone (patch) Middle Aged Prognosis Healthy Volunteers 030220 oncology & carcinogenesis testosterone biology.protein business Biomarkers |
| Zdroj: | Journal of Endocrinological Investigation. 44:321-326 |
| ISSN: | 1720-8386 |
| DOI: | 10.1007/s40618-020-01304-7 |
| Popis: | To meet clinicians’ request for adequate results and reliable reference ranges for testosterone, this study was planned with the aims (i) to verify the reliability of the reference interval for total testosterone (TT) declared by immunoassay manufacturer and adopted by laboratory, (ii) to compare results for serum TT obtained by immunoassay and LC–MS/MS and (iii) to verify if the cutoff values for low TT and measured free testosterone (FT), defined by Endocrine Society Guidelines for diagnosis of hypogonadism, are applicable to our study group. Sera from anonymous young/middle-aged male blood donors were selected for the study. TT was measured by immunoassay and LC–MS/MS. SHBG was measured by immunoassay and used with albumin concentration to calculate FT according to Vermeulen’s formula. The reference interval declared by the manufacturer and adopted by the lab was validated. The two methods for TT evaluation correlated very well. TT and FT lower limits at 5th and 2.5th percentile are below the cutoffs reported in the literature for the diagnosis of hypogonadism. The immunoassay currently used in our lab can be considered an adequate tool for TT, but it’s essential that clinical data agree with the biochemical ones, particularly in the presence of TT values between the lower limit of reference range and the cutoff values recommended by scientific societies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink