Electromembrane extraction of chlorprothixene, haloperidol and risperidone from whole blood and urine
| Autor: | Ying Dong, Chuixiu Huang, Ruiqin Zhu, Xiao Yu, Xinlin Li, Yuhang Shi, Sunzhao You |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Time Factors medicine.medical_treatment Chlorprothixene Urine 010402 general chemistry 01 natural sciences Biochemistry Analytical Chemistry Rats Sprague-Dawley Electricity Tandem Mass Spectrometry medicine Haloperidol Animals Humans Antipsychotic Whole blood Detection limit Risperidone Chromatography Chemistry 010401 analytical chemistry Organic Chemistry Reproducibility of Results Water Membranes Artificial General Medicine Repeatability 0104 chemical sciences Body Fluids Solvents Acids medicine.drug Antipsychotic Agents Chromatography Liquid |
| Zdroj: | Journal of chromatography. A. 1629 |
| ISSN: | 1873-3778 |
| Popis: | Separation of antipsychotic drugs from whole blood and urine is of great importance for clinic and forensic laboratories. In this work, chlorprothixene, haloperidol and risperidone representing the first and second generations of antipsychotic drugs were studied. Among them, chlorprothixene and risperidone were investigated for the first time by electromembrane extraction (EME). After the screening, 2-nitrophenyl octyl ether (NPOE) was used as the supported liquid membrane (SLM). The EME performance for spiked water (pH 2), whole blood and urine was tested and optimized individually. Using NPOE and 60 V, efficient EME was achieved from urine and whole blood with trifluoroacetic acid as the acceptor solution. The equilibrium time required for EME was dependent on the sample matrices. The steady-state of EME was reached in 30 min and 20 min for whole blood and urine, respectively. At steady-state, the EME recoveries of the targets from different sample matrices were satisfactory, and were in the range of 74%-100%. The proposed EME approach combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was evaluated using whole blood and urine. The obtained linearity was 1-200 ng mL-1, and the coefficient of determination (R2) was ≥ 0.9853 for haloperidol and ≥ 0.9936 for chlorprothixene and risperidone. The limit of detection (LOD) and accuracy for all the targets ranged from 0.2-0.6 ng mL-1 and 102%-110%, respectively, and the repeatability at low (1 ng mL-1), medium (10 ng mL-1) and high (200 ng mL-1) concentration was ≤ 12% (RSD). Finally, the validated approach was successfully used to determine chlorprothixene, risperidone and haloperidol in whole blood and urine from rats, which were treated with chlorprothixene, risperidone and haloperidol at low therapeutic dose, respectively. |
| Databáze: | OpenAIRE |
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