c-Abl tyrosine kinase interacts with MAVS and regulates innate immune response
| Autor: | Wei Shi, Kai Guan, Qing-Jun Ma, Yuan Yuan, Yang Xu, Yanhong Zhang, Hui Zhong, Congwen Wei, Ting Song, Zirui Zheng, Xiaozhong Cheng |
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| Rok vydání: | 2009 |
| Předmět: |
Innate immune response
c-Abl non-receptor tyrosine kinase Biophysics RIG1 signaling Biochemistry Receptor tyrosine kinase Cell Line Structural Biology hemic and lymphatic diseases Protein Interaction Mapping Genetics Humans Protein Interaction Domains and Motifs Phosphorylation Proto-Oncogene Proteins c-abl neoplasms Molecular Biology Adaptor Proteins Signal Transducing Gene knockdown Innate immune system biology NF-kappa B Signal transducing adaptor protein Interferon-beta Cell Biology Immunity Innate Mitochondrial antiviral signaling ROR1 biology.protein Cancer research Interferon Regulatory Factor-3 IRF3 Tyrosine kinase |
| Zdroj: | FEBS Letters. 584:33-38 |
| ISSN: | 0014-5793 |
| Popis: | The tyrosine kinase, c-Abl, plays important roles in many aspects of cellular function. Previous reports showed that c-Abl is involved in NF-κB signaling. However, the functions of c-Abl in innate immunity are still unknown. Here we demonstrate that the mitochondrial antiviral signaling (MAVS) protein can be physically associated with c-Abl in vivo and in vitro. MAVS interacted with c-Abl through its Card and TM domain. A phosphotyrosine-specific antibody indicated that MAVS was phosphorylated by c-Abl. Functional impairment of c-Abl attenuated MAVS or VSV induced type-I IFN production. Importantly, c-Abl knockdown in MCF7 cells displayed impaired MAVS-mediated NF-κB and IRF3 activation. Taken together, our results suggest that c-Abl modulates innate immune response through MAVS.Structured summaryMINT-7297498, MINT-7297511, MINT-7297557, MINT-7297574: MAVS (uniprotkb:Q7Z434) physically interacts (MI:0915) with c-Abl (uniprotkb:P00519) by anti tag coimmunoprecipitation (MI:0007)MINT-7297542: c-Abl (uniprotkb:P00519) physically interacts (MI:0915) with MAVS (uniprotkb:Q7Z434) by anti bait coimmunoprecipitation (MI:0006)MINT-7297526: c-Abl (uniprotkb:P00519) physically interacts (MI:0915) with MAVS (uniprotkb:Q7Z434) by far western blotting (MI:0047) |
| Databáze: | OpenAIRE |
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