Propionyl-L-carnitine prevents age-related myocardial remodeling in the rabbit
| Autor: | Marcella Marcellini, Claudio Pisano, Arianna Francesconi, Amedeo Ferlosio, Augusto Orlandi, Antonio Di Lascio, Luigi Giusto Spagnoli |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Pathology medicine.medical_specialty Aging Cardiotonic Agents Endothelium Vascular Cell Adhesion Molecule-1 Pharmacology Settore MED/08 - Anatomia Patologica Pharmacologic intervention Collagen Type I Cardiac fibroblasts Fibrosis Vascular cell adhesion molecule-1 Age related Carnitine medicine In Situ Nick-End Labeling Animals skin and connective tissue diseases Ventricular Remodeling business.industry Reverse Transcriptase Polymerase Chain Reaction Propionyl l carnitine Fibroblasts medicine.disease Endomyocardial Fibrosis Immunohistochemistry Disease Models Animal medicine.anatomical_structure sense organs Collagen Rabbits Cardiology and Cardiovascular Medicine business Signal Transduction |
| Zdroj: | Journal of cardiovascular pharmacology. 50(2) |
| ISSN: | 0160-2446 |
| Popis: | Age-related cardiac remodeling is characterized by changes in myocardial structure, which include fibrosis (ie, increased collagen concentration). The pathogenetic mechanisms of age-related cardiac changes and possible pharmacologic interventions are still a matter of investigation. A morphometric analysis of collagen accumulation was performed in Sirius Red-stained left ventricular sections of 3-month-old and 5-6-year-old animals after a 9-month period of propionyl-L-carnitine treatment (PLC; 120 mg Kg(-1) day(-1) per os); aged rabbits showed decreased interstitial collagen accumulation and no changes in cellularity and apoptotic rate compared to controls. Age-related expression of vascular cell adhesion molecule-1 (VCAM-1)-positive microvessels was also reduced in PLC-treated rabbits. In vitro, the 16-hour, 10-microM PLC treatment reduced collagen type 1 and VCAM-1 transcripts, which were investigated by reverse transcription-polymerase chain reaction, more markedly in cardiac fibroblasts from aged donors. In the latter, the anti-VCAM-1 antibody treatment was found to be associated with a reduction in collagen type I transcripts. Our results demonstrated that long-term PLC treatment partially prevents age-related interstitial remodeling and suggests that a more complex interstitial cell-to-cell signaling regulates senescent myocardium properties. |
| Databáze: | OpenAIRE |
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