GTPase-activating protein Elmod2 is essential for meiotic progression in mouse oocytes
| Autor: | Bo-Qun Xu, Jin-Wei Liu, Dong Zhang, Ya-Hong Liu, Zhixia Yang, Chun-Xiang Zhou, Li-Ya Shi, Bo Yuan, Rui-Chao Li, Xiaoyan Ying |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
GTPase-activating protein Biology Mitochondrion Chromosome segregation 03 medical and health sciences Adenosine Triphosphate GTP-binding protein regulators Meiosis GTP-Binding Proteins Chromosome Segregation Report medicine Animals Molecular Biology Anaphase Mice Inbred ICR Gene knockdown Ovary Cell Biology Aneuploidy Oocyte Mitochondria Cell biology Cytoskeletal Proteins 030104 developmental biology medicine.anatomical_structure Fertilization Gene Knockdown Techniques Oocytes Female Protein Binding Developmental Biology |
| Zdroj: | Cell Cycle. 16:852-860 |
| ISSN: | 1551-4005 1538-4101 |
| Popis: | Meiotic failure in oocytes is the major determinant of human zygote-originated reproductive diseases, the successful accomplishment of meiosis largely relay on the normal functions of many female fertility factors. Elmod2 is a member of the Elmod family with the strongest GAP (GTPase-activating protein) activity; although it was identified as a possible maternal protein, its actual physiologic role in mammalian oocytes has not been elucidated. Herein we reported that among Elmod family proteins, Elmod2 is the most abundant in mouse oocytes, and that inhibition of Elmod2 by specific siRNA caused severe meiotic delay and abnormal chromosomal segregation during anaphase. Elmod2 knockdown also significantly decreased the rate of oocyte maturation (to MII, with first polar body extrusion), and significantly greater numbers of Elmod2-knockdown MII oocytes were aneuploid. Correspondingly, Elmod2 knockdown dramatically decreased fertilization rate. To investigate the mechanism(s) involved, we found that Elmod2 knockdown caused significantly more abnormal mitochondrial aggregation and diminished cellular ATP levels; and we also found that Elmod2 co-localized and interacted with Arl2, a GTPase that is known to maintain mitochondrial dynamics and ATP levels in oocytes. In summary, we found that Elmod2 is the GAP essential to meiosis progression of mouse oocytes, most likely by regulating mitochondrial dynamics. |
| Databáze: | OpenAIRE |
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