Insulin and insulin mutants stimulate glucose uptake in rat adipocytes
| Autor: | Xintang Zhang, Shiyin Yao, Shangquan Zhu, Xinna Zhang, Yinghao Xu |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Chemistry Glucose uptake Insulin medicine.medical_treatment Mutant Biological activity General Biochemistry Genetics and Molecular Biology In vitro Endocrinology Basal (medicine) Internal medicine Insulin receptor substrate medicine General Agricultural and Biological Sciences General Environmental Science |
| Zdroj: | Science in China Series C: Life Sciences. 42:63-67 |
| ISSN: | 1862-2798 1006-9305 |
| DOI: | 10.1007/bf02881749 |
| Popis: | A simple method to determine the in vitro biological activity of insulin by measuring glucose uptake in the rat adipocytes is presented here. In the presence of insulin, the glucose uptake is 5-6 times more than the basal control. And the uptake of D-[3-(3)H]-glucose is linear as the logarithm of insulin concentration from 0.2 microg/L to 1.0 microg/L. Glucose and 3-O-methyl-glucose inhibit D-[3-(3)H]-glucose uptake into adipocytes. By this method, the in vitro biological activity of [B(2)-Lys]-insulin and [B(3)-Lys]-insulin was measured to be 61.6% and 154% respectively, relative to that of insulin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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