Characterization of a novel peripheral nervous system myelin protein (PMP-22/SR13)
| Autor: | A A Welcher, Eric M. Shooter, G J Snipes, Ueli Suter |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Aging
Molecular Sequence Data Central nervous system Schwann cell Biology Antibodies Immunoenzyme Techniques Myelin Peripheral myelin protein 22 Complementary DNA Gene expression medicine Animals Amino Acid Sequence RNA Messenger Messenger RNA Brain Articles Cell Biology Blotting Northern Sciatic Nerve Molecular biology Nerve Regeneration Rats Molecular Weight medicine.anatomical_structure Gene Expression Regulation Spinal Cord Peripheral nervous system Immunology Peptides Myelin Proteins |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.117.1.225 |
| Popis: | We have recently described a novel cDNA, SR13 (Welcher, A. A., U. Suter, M. De Leon, G. J. Snipes, and E. M. Shooter. 1991. Proc. Natl. Acad. Sci. USA. 88:7195-7199), that is repressed after sciatic nerve crush injury and shows homology to both the growth arrest-specific mRNA, gas3 (Manfioletti, G., M. E. Ruaro, G. Del Sal, L. Philipson, and C. Schneider, 1990. Mol. Cell Biol. 10:2924-2930), and to the myelin protein, PASII (Kitamura, K., M. Suzuki, and K. Uyemura. 1976. Biochim. Biophys. Acta. 455:806-816). In this report, we show that the 22-kD SR13 protein is expressed in the compact portion of essentially all myelinated fibers in the peripheral nervous system. Although SR13 mRNA was found in the central nervous system, no corresponding SR13 protein could be detected by either immunoblot analysis or by immunohistochemistry. Northern and immunoblot analysis of SR13 mRNA and protein expression during development of the peripheral nervous system reveal a pattern similar to other myelin proteins. Furthermore, we demonstrate by in situ mRNA hybridization on tissue sections and on individual nerve fibers that SR13 mRNA is produced predominantly by Schwann cells. We conclude that the SR13 protein is apparently exclusively expressed in the peripheral nervous system where it is a major component of myelin. Thus, we propose the name Peripheral Myelin Protein-22 (PMP-22) for the proteins and cDNA previously designated PASII, SR13, and gas3. |
| Databáze: | OpenAIRE |
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