Identification of new bioactive peptides from Kefir milk through proteopeptidomics: Bioprospection of antihypertensive molecules
| Autor: | Luciana Barbosa Coitinho, Bianca P. Campagnaro, Lucas Cunha Dias de Rezende, Andreia G. F. Friques, Brenna Lepaus Monteiro, Elisardo C. Vasquez, Thiago de Melo Costa Pereira, Loïc Quinton, Fernanda Gobbi Amorim, Edwin De Pauw, Ananda T. Dias |
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| Rok vydání: | 2019 |
| Předmět: |
Proteomics
Peptidyl-Dipeptidase A Pharmacology Models Biological 01 natural sciences Analytical Chemistry law.invention Probiotic Kefir 0404 agricultural biotechnology law Animals Shotgun proteomics Ace inhibition Antihypertensive Agents Binding Sites biology Chemistry 010401 analytical chemistry Angiotensin-converting enzyme 04 agricultural and veterinary sciences General Medicine 040401 food science Enzyme assay Protein Structure Tertiary 0104 chemical sciences Molecular Docking Simulation Plethysmography Milk Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Cattle Peptides Digestion Food Science |
| Zdroj: | Food Chemistry. 282:109-119 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2019.01.010 |
| Popis: | Kefir, a probiotic beverage prepared from fermented milk, has been associated with antihypertensive activity. However, the bioactive molecules responsible for this activity still remain unclear. Therefore, in this study we aim to evaluate the mechanisms of the antihypertensive effects of Kefir in the two-kidney one-clip hypertension model, and to bioprospect for bioactive peptides identified by proteomic methodologies. Treatment with Kefir was performed via gavage, and resulted in a 37 mmHg reduction in systolic arterial pressure and 19% inhibition of angiotensin converting enzyme (ACE) activity. For the proteopeptidomic study, the protein extract of Kefir beverage and non-fermented bovine milk were analysed by MALDI-TOF mass spectrometry, and their tryptic digestion products sequenced via Shotgun proteomics (Q-Exactive mass spectrometer). A list of 35 peptides with potential hypertensive activity due to ACE inhibition were identified. These results demonstrate the benefits of Kefir products, and may guide the design of new antihypertensive drugs. |
| Databáze: | OpenAIRE |
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