Occludin and claudin-1 concentrate in the midbody of immortalized mouse hepatocytes during cell division
| Autor: | Takashi Kojima, Yasuo Kokai, Yohichi Mochizuki, Hideki Chiba, Makoto Osanai, Michio Mori, Norimasa Sawada, Kazuhide Kuwahara |
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| Rok vydání: | 2001 |
| Předmět: |
0301 basic medicine
Histology DNA Complementary Cell division Mitosis Biology Occludin Transfection Zonula Occludens-2 Protein Connexins Tight Junctions 03 medical and health sciences Mice Claudin-1 Animals Telophase Claudin education education.field_of_study Mice Inbred C3H 030102 biochemistry & molecular biology Tight junction Membrane Proteins Phosphoproteins Cell biology Midbody 030104 developmental biology Microscopy Fluorescence Hepatocytes Zonula Occludens-1 Protein Connexin 32 Female Anatomy Cytokinesis |
| Zdroj: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 49(3) |
| ISSN: | 0022-1554 |
| Popis: | It has been believed that epithelial cells maintain tight junctions at all times, including during cell division, to provide a continuous epithelial seal. However, changes in localization of integral tight junction proteins during cell division have not been examined. In this study, using SV40-immortalized mouse hepatocytes transfected with human Cx32 cDNA, in which tight junction strands and the endogenous tight junction proteins occludin, claudin-1, ZO-1, and ZO-2 were induced, we examined changes in localization of the tight junction proteins at all stages of cell division. All tight junction proteins were present between mitotic cells and neighboring cells throughout cell division. In late telophase, the integral tight junction proteins occludin and claudin-1, but not the cytoplasmic proteins ZO-1 and ZO-2, were concentrated in the midbody between the daughter cells and were observed at cell borders between the daugher and neighboring cells. These results indicate that the integral tight junction proteins are regulated in a different manner from the cytoplasmic proteins ZO-1 and ZO-2 during cytokinesis. |
| Databáze: | OpenAIRE |
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