Effect of brain death on gene expression and tissue activation in human donor kidneys
| Autor: | Janneke Wiersema-Buist, Henri G. D. Leuvenink, Susan Fekken, Willem J. van Son, Jaap J. Homan van der Heide, Sijbrand Hofker, Joost A. B. van der Hoeven, Theo A. Schuurs, Willemijn N. Nijboer, Rutger J. Ploeg |
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| Přispěvatelé: | Surgery, Other departments, Faculteit Medische Wetenschappen/UMCG, Groningen Institute for Organ Transplantation (GIOT) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Gene Expression kidney transplantation Kidney ISCHEMIA/REPERFUSION INJURY Andrology Transforming Growth Factor beta Heat shock protein Living Donors Humans Medicine brain death HEAT-SHOCK PROTEINS HSP70 Heat-Shock Proteins adhesion molecules HEME OXYGENASE Chemokine CCL2 Kidney transplantation SUBARACHNOID HEMORRHAGE Aged Tumor Necrosis Factor-alpha Cell adhesion molecule business.industry TRANSPLANTATION Monocyte INDUCTION CYTOKINES Middle Aged ORGAN DONORS Intercellular Adhesion Molecule-1 Intercellular adhesion molecule medicine.disease Hsp70 Transplantation medicine.anatomical_structure inflammation heat shock proteins SURVIVAL RAT Female business |
| Zdroj: | Transplantation, 78(7), 978-986. Lippincott Williams and Wilkins Transplantation, 78(7), 978-986. LIPPINCOTT WILLIAMS & WILKINS |
| ISSN: | 0041-1337 |
| Popis: | Background. After kidney transplantation, decreased graft survival is seen in grafts from brain dead (BD) donors compared with living donors. This might result partly from a progressive nonspecific inflammation in the graft. In this study, we focused on the effects of BD on inflammatory response (adhesion molecules, leukocyte invasion, gene expression) and stress-related heat shock proteins in the human kidney. Research outcomes and clinical donor parameters were then linked to outcome data after transplantation.Methods. Kidney biopsy specimens and serum were obtained during organ retrieval from BD and living organ donor controls. Immunohistochemistry and semquantitative reverse transcriptase-polymerase chain reaction were performed on the biopsy specimens. Clinical and laboratory parameters from BD donors were recorded and connected to outcome data of the recipients of the kidneys studied.Results. After brain death, immunohistochemistry showed an increase of E-selectin (P Conclusions. The presence of interstitial leukocytes and the early adhesion molecule E-selectin in BD donor kidneys indicates an early-phase inflammatory process during organ retrieval. Elevated levels of monocyte chemotactic protein-1 and transforming growth factor-beta suggest a role for monocytes/macrophages in this phase. We suggest that BD causes a stress-related response against which protective heat shock proteins are formed in the future graft. This stress response may be too severe to be fully counteracted by elevated heat shock proteins. Which systemic and/or local factors trigger brain death-related graft injury is currently under investigation. |
| Databáze: | OpenAIRE |
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