Anti-Inflammatory and Autonomic Effects of Electroacupuncture in a Rat Model of Diet-Induced Obesity
| Autor: | Jian-Qing Song, Xiaoyan Jie, Dan Wang, Xu Li, Jian-Hua Wang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Necrosis alpha7 Nicotinic Acetylcholine Receptor Electroacupuncture medicine.medical_treatment Adipose tissue Autonomic Nervous System Diet High-Fat Proinflammatory cytokine Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Heart Rate Internal medicine Animals Humans Medicine Obesity Tumor Necrosis Factor-alpha business.industry Body Weight digestive oral and skin physiology food and beverages nutritional and metabolic diseases General Medicine Acetylcholine Rats Autonomic nervous system 030104 developmental biology Endocrinology Nicotinic agonist Complementary and alternative medicine Cholinergic Neurology (clinical) medicine.symptom business Acupuncture Points 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Acupuncture in Medicine. 36:103-109 |
| ISSN: | 1759-9873 0964-5284 |
| Popis: | Objective To study the effect of electroacupuncture (EA) on the cholinergic anti-inflammatory pathway (CAP) by measurement of vagal activity in rats with high-fat diet (HFD)-induced obesity. Methods Diet-induced obesity (DIO) was induced in 30 rats by feeding them a HFD for 12 weeks. A further 10 rats fed normal food comprised the lean diet (LD) control group. DIO rats were further subdivided into three groups that received a HFD only (HFD group, n=10), a HFD plus electroacupuncture (HFD+EA group, n=10) or a HFD plus minimal acupuncture (HFD+MA group, n=10). EA and MA treatments were continued for 8 weeks. Heart rate variability (HRV) was used to measure the function of the autonomic nervous system before and after treatment. ELISA was used to determine acetylcholine (ACh) and tumour necrosis factor (TNF)-α levels in the serum. Real-time PCR was used to assess the mRNA expression of α7-subtype nicotinic acetylcholine cholinergic receptors (α7nAChRs) and TNF-α in the mesenteric white adipose tissues (MWAT). Results EA but not MA significantly reduced rats’ bodyweight. No difference was found in the low frequency (LF), high frequency (HF) and the balance between LF and HF (LF/HF) components of HRV before treatment. After the EA intervention, HF was elevated and LF/HF was reduced in the HFD+EA group comparedwith the HFD group. TNF-α in the serum and MWAT were increased in the HFD group, but were reduced in the HFD+EA group. Furthermore, EA promoted expression of α7nAChRs and ACh in the MWAT. There was no difference between the HFD and HFD+MA groups for any indices. Conclusions EA enhanced vagal activity, promoted ACh release and activated α7nAChRs in the MWAT, leading to inhibition of proinflammatory cytokine production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|