Glycine betaine improves Listeria monocytogenes tolerance to desiccation on parsley leaves independent of the osmolyte transporters BetL, Gbu and OpuC

Autor: Cindy E. Morris, Roy D. Sleator, Colin Hill, Nicolas Dreux, Frédéric Carlin, Christophe Nguyen-The, Christine Albagnac
Přispěvatelé: Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork (UCC), Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2008
Předmět:
Osmotic shock
Colony Count
Microbial
Biological Transport
Active
Biology
medicine.disease_cause
GLYCINE BETAINE
Applied Microbiology and Biotechnology
Microbiology
HUMIDITE RELATIVE
03 medical and health sciences
chemistry.chemical_compound
Betaine
Listeria monocytogenes
Osmotic Pressure
medicine
COMPATIBLE SOLUTES
Osmotic pressure
Listeriosis
PRODUIT FRAIS
RELATIVE HUMIDITY
030304 developmental biology
Bacteriological Techniques
0303 health sciences
Microbial Viability
Virulence
LISTERIA MONOCYTOGENES
FOOD BORNE BACTERIA
030306 microbiology
Membrane Transport Proteins
food and beverages
Biological Transport
General Medicine
DESICCATION
FRESH PRODUCE
Culture Media
PERSIL
Plant Leaves
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
chemistry
Osmolyte
PROLINE
Glycine
Food Microbiology
Petroselinum
Osmoprotectant
Desiccation
Biotechnology
Zdroj: Journal of Applied Microbiology
Journal of Applied Microbiology, Wiley, 2008, 104 (4), pp.1221-1227. ⟨10.1111/j.1365-2672.2007.03623.x⟩
ISSN: 1365-2672
1364-5072
Popis: Aims: To investigate the effect of glycine betaine (GB) on the survival of Listeria monocytogenes on leaf surfaces under low relative humidity (RH). Methods and Results: The addition of GB (≥25 mmol l−1) improved the survival of L. monocytogenes under low RH on parsley leaves, thus suggesting that GB can improve the tolerance of L. monocytogenes to desiccation. Ten times less GB was needed to improve L. monocytogenes survival under low RH on nonbiological surfaces compared with parsley leaves, suggesting that, on the leaf surface, L. monocytogenes may have to compete for the available GB with autochthonous bacteria and/or the plant itself. Wild type and mutants carrying deletions in the three GB uptake systems, BetL, Gbu and OpuC, behaved similarly with and without added GB on parsley leaves (P > 0·05). In addition, preaccumulation of GB, triggered by osmotic stress prior to inoculation, failed to improve survival under low RH compared with osmotic stress without GB accumulation. Conclusions: Exogenous GB had a protective effect on L. monocytogenes cells from desiccation during survival on parsley leaves. This effect was independent of intracellular GB accumulation by the known uptake systems. Significance and Impact of the Study: Presence of GB could improve the survival of L. monocytogenes to desiccation on leaf surfaces and nonbiological surfaces.
Databáze: OpenAIRE
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