Candidavirulence and ethanol-derived acetaldehyde production in oral cancer and non-cancer subjects
| Autor: | Eric C. Reynolds, Adlin N. Ramdzan, David Wiesenfeld, Neil M O'Brien-Simpson, Spas D. Kolev, Ali D. Alnuaimi, Michael McCullough |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Acetaldehyde Phospholipase Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Candidiasis Oral medicine Humans Candida albicans General Dentistry Carcinogen Candida Mouth neoplasm chemistry.chemical_classification Ethanol biology Biofilm Cancer biology.organism_classification medicine.disease 030104 developmental biology Enzyme Otorhinolaryngology chemistry Biofilms Case-Control Studies 030220 oncology & carcinogenesis Female Mouth Neoplasms |
| Zdroj: | Oral Diseases. 22:805-814 |
| ISSN: | 1354-523X |
| DOI: | 10.1111/odi.12565 |
| Popis: | Objectives To compare biofilm-forming ability; hydrolytic enzymes and ethanol-derived acetaldehyde production of oral Candida isolated from the oral cancer and matched non-oral cancer patients. Material and Methods Fungal biofilms were grown in RPMI-1640 medium and biofilm- mass and -activity were assessed using crystal-violet staining and XTT-salt reduction assays, respectively. Phospholipase, proteinase and esterase production was measured using agar plate method, while fungal acetaldehyde production was assessed via gas-chromatography. Results Candida isolated from oral cancer patients demonstrated significantly higher biofilm mass (p=0.031), biofilm metabolic activity (p |
| Databáze: | OpenAIRE |
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