Caenorhabditis elegans Fibroblast Growth Factor Receptor Signaling Can Occur Independently of the Multi-Substrate Adaptor FRS2
| Autor: | Daniel C. Bennett, Te-Wen Lo, Michael J. Stern, S. Jay Goodman |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
MAPK/ERK pathway
animal structures Investigations Receptor tyrosine kinase Cell Movement Genetics Animals Caenorhabditis elegans Adaptor Proteins Signal Transducing GRB2 Adaptor Protein Binding Sites biology fungi Membrane Proteins Proteins Receptor Protein-Tyrosine Kinases Signal transducing adaptor protein biology.organism_classification Receptors Fibroblast Growth Factor Cell biology Genes Fibroblast growth factor receptor embryonic structures biology.protein GRB2 Signal transduction Protein Binding Signal Transduction |
| Zdroj: | Genetics. 185:537-547 |
| ISSN: | 1943-2631 |
| Popis: | The components of receptor tyrosine kinase signaling complexes help to define the specificity of the effects of their activation. The Caenorhabditis elegans fibroblast growth factor receptor (FGFR), EGL-15, regulates a number of processes, including sex myoblast (SM) migration guidance and fluid homeostasis, both of which require a Grb2/Sos/Ras cassette of signaling components. Here we show that SEM-5/Grb2 can bind directly to EGL-15 to mediate SM chemoattraction. A yeast two-hybrid screen identified SEM-5 as able to interact with the carboxy-terminal domain (CTD) of EGL-15, a domain that is specifically required for SM chemoattraction. This interaction requires the SEM-5 SH2-binding motifs present in the CTD (Y1009 and Y1087), and these sites are required for the CTD role of EGL-15 in SM chemoattraction. SEM-5, but not the SEM-5 binding sites located in the CTD, is required for the fluid homeostasis function of EGL-15, indicating that SEM-5 can link to EGL-15 through an alternative mechanism. The multi-substrate adaptor protein FRS2 serves to link vertebrate FGFRs to Grb2. In C. elegans, an FRS2-like gene, rog-1, functions upstream of a Ras/MAPK pathway for oocyte maturation but is not required for EGL-15 function. Thus, unlike the vertebrate FGFRs, which require the multi-substrate adaptor FRS2 to recruit Grb2, EGL-15 can recruit SEM-5/Grb2 directly. |
| Databáze: | OpenAIRE |
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