Stimulation of poly(ADP-ribose) synthesis by free radicals in C3H10T1/2 cells: relationship with NAD metabolism and DNA breakage
| Autor: | Lautier D, Alaoui Jamali Ma, Guy G. Poirier, Boudreau A, Poirier D, Castonguay A |
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| Rok vydání: | 1990 |
| Předmět: |
Poly Adenosine Diphosphate Ribose
Xanthine Oxidase Free Radicals Dna breakage Radical NAD metabolism Stimulation Tritium Biochemistry Xanthine Cell Line chemistry.chemical_compound Mice Superoxides Ribose Animals Xanthine oxidase Molecular Biology Chromatography High Pressure Liquid Nucleoside Diphosphate Sugars Phosphoric Diester Hydrolases Cell Biology Hydrogen Peroxide Hydrogen-Ion Concentration Alkaline Phosphatase NAD Molecular biology Clone Cells chemistry Gamma Rays Xanthines Benzamides DNA DNA Damage |
| Zdroj: | Biochemistry and cell biology = Biochimie et biologie cellulaire. 68(3) |
| ISSN: | 0829-8211 |
| Popis: | We have studied the effect of H2O2 and O2− produced by xanthine and xanthine oxidase on NAD catabolism, poly(ADP-ribose) synthesis, and production of DNA single-strand breaks in C3H10T1/2 cells. The results show a correlation between the induction of DNA single-strand breaks, the decrease of NAD pool, and the accumulation of polymer. New techniques, based on affinity chromatography and reversed-phase high pressure liquid chromatography, have allowed an accurate determination of polymer contents and showed a 20-fold stimulation of polymer biosynthesis induced by active oxygen species. Inhibition experiments performed with 3-aminobenzamide have shown that the decrease in NAD levels after exposure of cells to active oxygen species was caused by stimulation of poly(ADP-ribosyl)ation and of another cellular process.Key words: poly(ADP-ribose), free radicals, C3H10T1/2 cells, DNA breakage, NAD catabolism. |
| Databáze: | OpenAIRE |
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