Diverse biological effects of glycosyltransferase genes from Tartary buckwheat

Autor: Hui Chen, Renyu Deng, Jiaqi Shi, Bingbing Lv, Guanlan Shi, Haixia Zhao, Qi Wu, Qixin Dong, Yunji Huang, Chenglei Li, Simon Stael, Panfeng Yao, Qi Li
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Arabidopsis
AUXIN
Plant Science
ANTHOCYANINS
01 natural sciences
Anthocyanins
chemistry.chemical_compound
Rutin
FUNCTIONAL-CHARACTERIZATION
lcsh:Botany
Arabidopsis thaliana
TEMPERATURE
Conserved Sequence
Phylogeny
Plant Proteins
chemistry.chemical_classification
biology
Fagopyrum tataricum
food and beverages
Plants
Genetically Modified
lcsh:QK1-989
UDP-GLYCOSYLTRANSFERASES
Fagopyrum
Research Article
EXPRESSION
Development
Genes
Plant
Flavonoids glycosyltransferase
3-O-GLUCOSYLTRANSFERASE
03 medical and health sciences
Auxin
Botany
Tartary buckwheat
ACCUMULATION
Flavonoids
IDENTIFICATION
fungi
Biology and Life Sciences
Glycosyltransferases
Sequence Analysis
DNA
biology.organism_classification
030104 developmental biology
Flavonoid biosynthesis
chemistry
Anthocyanin
010606 plant biology & botany
Zdroj: BMC Plant Biology, Vol 19, Iss 1, Pp 1-15 (2019)
BMC Plant Biology
BMC PLANT BIOLOGY
ISSN: 1471-2229
DOI: 10.1186/s12870-019-1955-z
Popis: Background Tartary buckwheat (Fagopyrum tataricum) is an edible cereal crop whose sprouts have been marketed and commercialized for their higher levels of anti-oxidants, including rutin and anthocyanin. UDP-glucose flavonoid glycosyltransferases (UFGTs) play an important role in the biosynthesis of flavonoids in plants. So far, few studies are available on UFGT genes that may play a role in tartary buckwheat flavonoids biosynthesis. Here, we report on the identification and functional characterization of seven UFGTs from tartary buckwheat that are potentially involved in flavonoid biosynthesis (and have varying effects on plant growth and development when overexpressed in Arabidopsis thaliana.) Results Phylogenetic analysis indicated that the potential function of the seven FtUFGT proteins, FtUFGT6, FtUFGT7, FtUFGT8, FtUFGT9, FtUFGT15, FtUFGT40, and FtUFGT41, could be divided into three Arabidopsis thaliana functional subgroups that are involved in flavonoid biosynthesis of and anthocyanin accumulation. A significant positive correlation between FtUFGT8 and FtUFGT15 expression and anthocyanin accumulation capacity was observed in the tartary buckwheat seedlings after cold stress. Overexpression in Arabidopsis thaliana showed that FtUFGT8, FtUFGT15, and FtUFGT41 significantly increased the anthocyanin content in transgenic plants. Unexpectedly, overexpression of FtUFGT6, while not leading to enhanced anthocyanin accumulation, significantly enhanced the growth yield of transgenic plants. When wild-type plants have only cotyledons, most of the transgenic plants of FtUFGT6 had grown true leaves. Moreover, the growth speed of the oxFtUFGT6 transgenic plant root was also significantly faster than that of the wild type. At later growth, FtUFGT6 transgenic plants showed larger leaves, earlier twitching times and more tillers than wild type, whereas FtUFGT15 showed opposite results. Conclusions Seven FtUFGTs were isolated from tartary buckwheat. FtUFGT8, FtUFGT15, and FtUFGT41 can significantly increase the accumulation of total anthocyanins in transgenic plants. Furthermore, overexpression of FtUFGT6 increased the overall yield of Arabidopsis transgenic plants at all growth stages. However, FtUFGT15 shows the opposite trend at later growth stage and delays the growth speed of plants. These results suggested that the biological function of FtUFGT genes in tartary buckwheat is diverse. Electronic supplementary material The online version of this article (10.1186/s12870-019-1955-z) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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