Duplex Real-Time PCR Method for the Differentiation of Cronobacter sakazakii and Cronobacter malonaticus
| Autor: | Zhigang Cui, Xiaofang Li, Yibing Huang, Jinghua Cui, Biao Kan, Xiaoli Du |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology 030106 microbiology Cronobacter malonaticus Real-Time Polymerase Chain Reaction biology.organism_classification 16S ribosomal RNA Microbiology Cronobacter sakazakii Infant Formula Cronobacter muytjensii 03 medical and health sciences Cronobacter Real-time polymerase chain reaction Cronobacter turicensis Cronobacter dublinensis RNA Ribosomal 16S Humans Food Science |
| Zdroj: | Journal of Food Protection. 80:50-56 |
| ISSN: | 0362-028X |
| DOI: | 10.4315/0362-028x.jfp-16-171 |
| Popis: | Cronobacter sakazakii and Cronobacter malonaticus are the most common species of Cronobacter, so it is necessary to detect the two species as soon as possible in surveillance programs. We developed a real-time PCR method for identifying C. sakazakii and C. malonaticus from the genus Cronobacter. In this study, the two pairs of primers and probes were designed, targeting 16S rRNA and fusA, respectively. The specificity of the real-time PCR assay was validated with 112 strains of Cronobacter, including 56 C. sakazakii, 32 C. malonaticus, 16 Cronobacter dublinensis, 6 Cronobacter turicensis, and 2 Cronobacter muytjensii. The results showed that C. sakazakii and C. malonaticus were all correctly identified, consistent with the results of another method by analyzing the clustering of the fusA sequence. The detection limit for pure culture was 102 CFU/ml and 103 CFU/g for artificially contaminated rehydrated powdered infant formula. Therefore, the developed real-time PCR was a rapid, sensitive, and rel... |
| Databáze: | OpenAIRE |
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