Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activity
| Autor: | Clayton A. Smith, Robert W. Storms, O M Colvin, L Shah, A P Trujillo, Susan M. Ludeman, J B Springer |
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| Rok vydání: | 1999 |
| Předmět: |
Boron Compounds
Population CD34 Aldehyde dehydrogenase Cell Separation Biology Substrate Specificity Flow cytometry Colony-Forming Units Assay medicine Humans Progenitor cell Coloring Agents education education.field_of_study Multidisciplinary medicine.diagnostic_test Infant Newborn Biological Sciences Aldehyde Dehydrogenase Fetal Blood Flow Cytometry Hematopoietic Stem Cells Molecular biology Haematopoiesis Verapamil biology.protein Stem cell K562 Cells K562 cells |
| Zdroj: | Proceedings of the National Academy of Sciences. 96:9118-9123 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Because hematopoietic stem cells are rich in aldehyde dehydrogenase (ALDH) activity, we developed a fluorescent substrate for ALDH, termed BODIPY aminoacetaldehyde (BAAA), and tested its potential for isolating primitive human hematopoietic cells. A population of cells with low orthogonal light scattering and bright fluorescence intensity (SSCloALDHbrcells) could be readily fractionated from human umbilical cord blood cells costained with BAAA and the multidrug-resistance inhibitor verapamil. The SSCloALDHbrpopulation was depleted of lineage-committed cells, 40–90% pure for CD34+CD38lo/−cells, and enriched 50- to 100-fold for primitive hematopoietic progenitors detected in short- and long-term culture analyses. Together, these observations indicate that fractionating human hematopoietic stem cells on the basis of ALDH activity using BAAA is an effective method for isolating primitive human hematopoietic progenitors. This technique may be useful for isolating stem cells from other tissues as well. |
| Databáze: | OpenAIRE |
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