Effects of a high-fat diet and strain on hypothalamic gene expression in rats
| Autor: | David A. York, Andrea O. Schaffhauser, H.D. Braymer, George A. Bray, Abram M. Madiehe |
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| Rok vydání: | 2002 |
| Předmět: |
Leptin
Male medicine.medical_specialty Corticotropin-Releasing Hormone Endocrinology Diabetes and Metabolism Hypothalamus Medicine (miscellaneous) Gene Expression Biology Weight Gain Eating Endocrinology Species Specificity Internal medicine Gene expression medicine Receptor Serotonin 5-HT2C Animals Insulin Neuropeptide Y Northern blot Obesity RNA Messenger Receptor Public Health Environmental and Occupational Health Organ Size medicine.disease Neuropeptide Y receptor Dietary Fats Rats Receptors Neuropeptide Y Receptors Serotonin Serotonin Corticosterone Food Science Hormone |
| Zdroj: | Obesity research. 10(11) |
| ISSN: | 1071-7323 |
| Popis: | Objective: This study was designed to investigate whether dietary fat and genetic background might differentially alter the expression of hypothalamic genes involved in food intake. Research Methods and Procedures: Three-month-old Osborne-Mendel (OM) and S5B/Pl rats were fed either a high-fat or a low-fat diet for 14 days. mRNA for neuropeptide Y (NPY), corticotrophin-releasing hormone, NPY Y-1 receptor and Y-5 receptor, and serotonin 2c (5-HT2c) receptors were measured using Northern blotting or ribonuclease protection assays. Results: OM rats showed an increased expression of NPY and corticotrophin-releasing hormone compared with S5B/Pl rats. The expression of NPY-Y1 and -Y5 receptor mRNA was significantly higher in the hypothalamus of OM rats compared with S5B/Pl rats. The expression of 5HT-2c receptor mRNA was significantly reduced in both strains of rats eating a high-fat diet when compared with the animals eating the low-fat diet. Discussion: These data suggest that over activity of the NPY system may contribute to the development of obesity in OM rats and that expression of the 5HT-2c receptor gene may be modulated by dietary fat. |
| Databáze: | OpenAIRE |
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