Efficient replication of severe acute respiratory syndrome coronavirus in mouse cells is limited by murine angiotensin-converting enzyme 2
| Autor: | John L. Sullivan, Natalya Vasilieva, Mohan Somasundaran, Michael Farzan, Michael Moore, Thomas C. Greenough, Wenhui Li, Hyeryun Choe |
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| Rok vydání: | 2004 |
| Předmět: |
viruses
Immunology Peptidyl-Dipeptidase A medicine.disease_cause Severe Acute Respiratory Syndrome Virus Replication Microbiology 3T3 cells Virus Cell Line Mice Nidovirales Virology Endopeptidases medicine Coronaviridae Animals Humans skin and connective tissue diseases Coronavirus biology 3T3 Cells biology.organism_classification Rats Virus-Cell Interactions medicine.anatomical_structure Viral replication Severe acute respiratory syndrome-related coronavirus Cell culture Insect Science Angiotensin-converting enzyme 2 hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Virology |
| ISSN: | 0022-538X |
| Popis: | Replication of viruses in species other than their natural hosts is frequently limited by entry and postentry barriers. The coronavirus that causes severe acute respiratory syndrome (SARS-CoV) utilizes the receptor angiotensin-converting enzyme 2 (ACE2) to infect cells. Here we compare human, mouse, and rat ACE2 molecules for their ability to serve as receptors for SARS-CoV. We found that, compared to human ACE2, murine ACE2 less efficiently bound the S1 domain of SARS-CoV and supported less-efficient S protein-mediated infection. Rat ACE2 was even less efficient, at near background levels for both activities. Murine 3T3 cells expressing human ACE2 supported SARS-CoV replication, whereas replication was less than 10% as efficient in the same cells expressing murine ACE2. These data imply that a mouse transgenically expressing human ACE2 may be a useful animal model of SARS. |
| Databáze: | OpenAIRE |
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