Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism
| Autor: | Joseph F. Bambico, Claro N. Mingala, Nathaniel D. Antonio, Ralph Lorenz R. Apilado, Jessica G. Manalaysay |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Scrotal Hernia Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Swine Mutant BAX Gene lcsh:Animal biochemistry Biology Gene Article law.invention 03 medical and health sciences 0302 clinical medicine law lcsh:QP501-801 Polymerase Polymerase chain reaction lcsh:SF1-1100 Molecular biology DNA extraction Animal Biotechnology 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Screening Animal Science and Zoology Gene polymorphism lcsh:Animal culture Restriction fragment length polymorphism Food Science |
| Zdroj: | Asian-Australasian Journal of Animal Sciences, Vol 30, Iss 2, Pp 262-266 (2017) Asian-Australasian Journal of Animal Sciences |
| ISSN: | 1976-5517 1011-2367 |
| Popis: | Objective This study was conducted to screen scrotal hernia in domesticated swine from selected breeders in the Philippines. This defect is associated with a cytosine to thymine mutation in the BCL-2 associated X protein (BAX) gene of swine. Methods Genetic screening was done by DNA extraction followed by amplification and digestion using polymerase chain reaction-restriction fragment length polymorphism, amplifying the 416 bp region of the BAX gene that was subjected to digestion using the Ear I enzyme. Sequencing was also conducted to validate the results. Results Results revealed that out of 538 samples tested, 411 (76.4%) of the samples were found to be normal whereas the remaining were carriers of the mutation in which 80 (14.9%) were heterozygous mutants and 47 (8.7%) were homozygous mutants. Pietrain breed was found to have the highest incidence. Conclusion Having a scrotal hernia eliminates the chances of using the boar as a breeder stock because the following generations arising from it would most likely exhibit herniation. It is therefore advised to establish a genetic screening method for Scrotal Hernia in the Philippines to eliminate the negative gene from the herd. |
| Databáze: | OpenAIRE |
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