Two-dimensional liquid chromatography (LC) of phenolic compounds from the shoots of Rubus idaeus ‘Glen Ample’ cultivar variety
| Autor: | Marta Kula, Daniel Głód, Mirosława Krauze-Baranowska |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Flavonols
Clinical Biochemistry Pharmaceutical Science Atmospheric-pressure chemical ionization 01 natural sciences Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Ellagic Acid Phenols Drug Discovery Cultivar Chromatography High Pressure Liquid Spectroscopy chemistry.chemical_classification Chromatography biology Plant Extracts 010401 analytical chemistry Polyphenols 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Hydrolyzable Tannins 0104 chemical sciences chemistry Polyphenol Shoot Rubus Plant Shoots Ellagic acid |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 121:99-106 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2015.12.047 |
| Popis: | In this study the application of two-dimensional LC (2D LC) for qualitative analysis of polyphenols and simple phenols in the shoots of Rubus idaeus 'Glen Ample' variety is presented. In the preliminary analysis, the methanol extract of the shoots was analyzed by one-dimensional LC. One-dimensional LC separation profiles of phenolics from R. idaeus 'Glen Ample' shoots were dependent on column type, mobile phase composition and gradient program used. Two-dimensional LC system was built from connecting an octadecyl C-18 silica column in the first dimension and pentafluorophenyl column in the second dimension, coupled with DAD and MS (ESI, APCI, DUIS ionization) detectors. A total of 34 phenolic compounds belonging to the groups of phenolic acids, ellagitannins, flavan-3-ols, flavonols and ellagic acid conjugates were identified in the shoots of R. idaeus 'Glen Ample'. The established 2D LC method offers an effective tool for analysis of phenolics present in Rubus species. |
| Databáze: | OpenAIRE |
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