Protective Effects of Timosaponin AIII against UVB-Radiation Induced Inflammation and DNA Injury in Human Epidermal Keratinocytes
| Autor: | Sungwook Chae, Ki Mo Kim, Se Kyu Park, Hyoung Seok Shin, A-Rang Im |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Keratinocytes Cell cycle checkpoint DNA repair Cell Survival Ultraviolet Rays Photoaging Anti-Inflammatory Agents Pharmaceutical Science Radiation-Protective Agents 03 medical and health sciences Anemarrhena asphodeloides 0302 clinical medicine medicine Humans Protein kinase A Cells Cultured Pharmacology integumentary system biology Chemistry NF-kappa B General Medicine Saponins medicine.disease biology.organism_classification Proliferating cell nuclear antigen Transcription Factor AP-1 030104 developmental biology Matrix Metalloproteinase 9 Cyclooxygenase 2 030220 oncology & carcinogenesis biology.protein Cancer research Tumor necrosis factor alpha Steroids Mitogen-Activated Protein Kinases DNA Damage Signal Transduction |
| Zdroj: | Biologicalpharmaceutical bulletin. 42(9) |
| ISSN: | 1347-5215 |
| Popis: | UVB radiation changes several photoaging pathway in the body, thereby prompting skin injury. Besides, chronic UVB radiation leads to photoaging, sustained immunosuppression, and photocarcinogenesis. We investigated the protective effect of Timosaponin AIII (TA-III), a naturally occurring steroidal saponin separated from Anemarrhena asphodeloides, against UVB-induced invasive properties of human epidermal keratinocytes (HEKs) and human dermal fibroblasts (HDF). No cytotoxicity was observed up to 50 nM concentration of TA-III. Similarly, TA-III inhibited UVB-induced cyclooxygenase-2 (COX-2), matrix metalloproteinase-9 (MMP-9) transcription level and protein expression in a dose-dependent manner at non-cytotoxic dose. Further, TA-III decreased UVB-induced invasion in primary skin cells. Additionally, TA-III suppressed UVB-stimulates mitogen-activated protein kinase (MAPK) signaling, activator protein-1 (AP-1) and nuclear factor kappa B (NF-κB) activation, thereby preventing the overexpression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and COX-2 in human epidermal keratinocytes cells. Furthermore, TA-III prevented UVB-mediated formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG) and activation of DNA repair enzymes and, cell cycle arrest genes like as proliferating cell nuclear antigen (PCNA), structural maintenance of chromosomes protein 1 (SMC1). This results support that understanding into the molecular action of TA-III, which can be useful for developing photoprotective agents. |
| Databáze: | OpenAIRE |
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