Spatial gradients of blood vessels and hematopoietic stem and progenitor cells within the marrow cavities of the human skeleton

Autor: Vincent A. Bourke, Christopher J Watchman, Arnaud Dieudonné, John D. Reith, Wesley E. Bolch, Marda Jorgensen
Přispěvatelé: Breton, Céline, Department of Radiation Oncology, University of Arizona, Department of Pathology, Immunology and Laboratory Medicine, University of North Florida [Jacksonville] (UNF), Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes (LITIS), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA), Nuclear & Radiological Engineering and Biomedical Engineering, University of Florida [Gainesville] (UF), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)
Rok vydání: 2009
Předmět:
Hematopoiesis and Stem Cells
Immunology
Population
CD34
Antigens
CD34
Bone Marrow Cells
Cell Count
Ribs
Biology
Biochemistry
MESH: Ilium
MESH: Hematopoietic Stem Cells
Ilium
MESH: Lumbar Vertebrae
03 medical and health sciences
0302 clinical medicine
Bone Marrow
medicine
Humans
Progenitor cell
education
030304 developmental biology
0303 health sciences
education.field_of_study
MESH: Humans
Lumbar Vertebrae
MESH: Cell Count
MESH: Bone Marrow Cells
MESH: Antigens
CD34
Cell Biology
Hematology
Anatomy
[SDV.ETH] Life Sciences [q-bio]/Ethics
Hematopoietic Stem Cells
MESH: Ribs
digestive system diseases
[SDV.ETH]Life Sciences [q-bio]/Ethics
Cell biology
MESH: Proto-Oncogene Proteins c-kit
Proto-Oncogene Proteins c-kit
Haematopoiesis
medicine.anatomical_structure
030220 oncology & carcinogenesis
MESH: Bone Marrow
Bone marrow
Stem cell
Cancellous bone
Blood vessel
Zdroj: Blood
Blood, American Society of Hematology, 2009, 114 (19), pp.4077-80. ⟨10.1182/blood-2008-12-192922⟩
ISSN: 1528-0020
0006-4971
DOI: 10.1182/blood-2008-12-192922
Popis: This report evaluates the spatial profile of blood vessel fragments (BVFs) and CD34+ and CD117+ hematopoietic stem and progenitor cells (HSPCs) in human cancellous bone. Bone specimens were sectioned, immunostained (anti-CD34 and anti-CD117), and digitally imaged. Immunoreactive cells and vessels were then optically and morphometrically identified and labeled on the corresponding digital image. The distance of each BVF, or CD34+ or CD117+ HSPC to the nearest trabecular surface was measured and binned in 50-μm increments. The relative concentration of HSPCs and BVFs within cancellous marrow was observed to diminish with increasing distance in the marrow space. On average, 50% of the CD34+ HSPC population, 60% of the CD117+ HSPC population, and 72% of the BVFs were found within 100 μm of the bone surfaces. HSPCs were also found to exist in close proximity to BVFs, which supports the notion of a shared HSPC and vessel spatial niche.
Databáze: OpenAIRE
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