Concurrent use of transgenic plants expressing a single and two Bacillus thuringiensis genes speeds insect adaptation to pyramided plants
| Autor: | Hilda L. Collins, Jun Cao, Anthony M. Shelton, Jian-Zhou Zhao, Sarah L. Bates, Richard T. Roush, Elizabeth D. Earle |
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| Rok vydání: | 2005 |
| Předmět: |
media_common.quotation_subject
Bacterial Toxins Adaptation Biological Brassica Insect Genetically modified crops Moths Insecticide Resistance Hemolysin Proteins Bacterial Proteins Bacillus thuringiensis Animals Computer Simulation Selection Genetic Gene media_common Population Density Analysis of Variance Multidisciplinary Diamondback moth Bacillus thuringiensis Toxins biology business.industry fungi food and beverages Plutella Models Theoretical Biological Sciences Plants Genetically Modified biology.organism_classification Survival Analysis Biotechnology Endotoxins Cry1Ac Larva business |
| Zdroj: | Proceedings of the National Academy of Sciences. 102:8426-8430 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Transgenic plants expressing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) were grown on over 13 million ha in the United States and 22.4 million ha worldwide in 2004. Preventing or slowing the evolution of resistance by insects (“resistance management”) is critical for the sustainable use of Bt crops. Plants containing two dissimilar Bt toxin genes in the same plant (“pyramided”) have the potential to delay insect resistance. However, the advantage of pyramided Bt plants for resistance management may be compromised if they share similar toxins with single-gene plants that are deployed simultaneously. We tested this hypothesis using a unique model system composed of broccoli plants transformed to express different Cry toxins (Cry1Ac, Cry1C, or both) and a synthetic population of the diamondback moth ( Plutella xylostella ) carrying genes for resistance to Cry1Ac and Cry1C at frequencies of ≈0.10 and 0.34, respectively. After 24–26 generations of selection in the greenhouse, the concurrent use of one- and two-gene plants resulted in control failure of both types of Bt plants. When only two-gene plants were used in the selection, no or few insects survived on one- or two-gene Bt plants, indicating that concurrent use of transgenic plants expressing a single and two Bt genes will select for resistance to two-gene plants more rapidly than the use of two-gene plants alone. The results of this experiment agree with the predictions of a Mendelian deterministic simulation model and have important implications for the regulation and deployment of pyramided Bt plants. |
| Databáze: | OpenAIRE |
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