An mRNA-mRNA Interaction Couples Expression of a Virulence Factor and Its Chaperone in Listeria monocytogenes
| Autor: | Jörgen Johansson, Ciarán Condon, Sylvain Durand, Laty A. Cahoon, Stefanie S. Sandberg, Birgitte H. Kallipolitis, Patrik Rydén, Karolis Vaitkevicius, Nancy E. Freitag, Xijia Liu, Dmitriy Ignatov |
|---|---|
| Přispěvatelé: | Umeå University, Expression Génétique Microbienne (EGM (UMR_8261 / FRE_3630)), Institut de biologie physico-chimique (IBPC (FR_550)), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), ANR-15-CE11-0009,ARNr-QC,La maturation de l'ARN ribosomique et son lien avec le contrôle de qualité de l'assemblage du ribosome(2015), ANR-16-CE12-0002,BaRR,Les ARNs régulateurs et leur rôle dans la dégradation des ARNm chez Bacillus subtilis(2016) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Untranslated region RNA Stability Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology) Molecular Biology Microbiology Biochemistry or Biopharmacy) medicine.disease_cause Virulence factor 0302 clinical medicine Gene expression RNA structure 3' Untranslated Regions lcsh:QH301-705.5 biology Virulence Temperature RNA thermosensor Peptidylprolyl Isomerase 3. Good health Cell biology RNA Bacterial listeriolysin O PrfA Virulence Factors Models Biological General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Ribonucleases Listeria monocytogenes [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] medicine small RNA RNA Messenger Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi) molekylärbiologi mikrobiologi biokemi eller biofarmaci) Gene Library Messenger RNA Base Sequence RNA Gene Expression Regulation Bacterial 5′ untranslated regions [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology 030104 developmental biology lcsh:Biology (General) Chaperone (protein) biology.protein Cold Shock Proteins and Peptides PrsA2 chaperone 5' Untranslated Regions Ribosomes 030217 neurology & neurosurgery mRNA-mRNA interaction Molecular Chaperones |
| Zdroj: | Cell Reports Cell Reports, Elsevier Inc, 2020, 30 (12), pp.4027-4040.e7. ⟨10.1016/j.celrep.2020.03.006⟩ Cell Reports, Vol 30, Iss 12, Pp 4027-4040.e7 (2020) Cell reports Ignatov, D, Vaitkevicius, K, Durand, S, Cahoon, L, Sandberg, S S, Liu, X, Kallipolitis, B H, Rydén, P, Freitag, N, Condon, C & Johansson, J 2020, ' An mRNA-mRNA Interaction Couples Expression of a Virulence Factor and Its Chaperone in Listeria monocytogenes ', Cell Reports, vol. 30, no. 12, pp. 4027-4040 . https://doi.org/10.1016/j.celrep.2020.03.006 |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2020.03.006⟩ |
| Popis: | SUMMARY Bacterial pathogens often employ RNA regulatory elements located in the 5′ untranslated regions (UTRs) to control gene expression. Using a comparative structural analysis, we examine the structure of 5′ UTRs at a global scale in the pathogenic bacterium Listeria monocytogenes under different conditions. In addition to discovering an RNA thermoswitch and detecting simultaneous interaction of ribosomes and small RNAs with mRNA, we identify structural changes in the 5′ UTR of an mRNA encoding the post-translocation chaperone PrsA2 during infection conditions. We demonstrate that the 5′ UTR of the prsA2 mRNA base pairs with the 3′ UTR of the full-length hly mRNA encoding listeriolysin O, thus preventing RNase J1-mediated degradation of the prsA2 transcript. Mutants lacking the hly-prsA2 interaction exhibit reduced virulence properties. This work highlights an additional level of RNA regulation, where the mRNA encoding a chaperone is stabilized by the mRNA encoding its substrate. In Brief By using a comparative structural analysis method targeting 5′ untranslated regions, Ignatov et al. identify different RNA-based regulatory mechanisms such as an RNA thermoswitch, a small RNA (sRNA)-mRNA interaction, and strikingly, an mRNA-mRNA interaction. Graphical Abstract |
| Databáze: | OpenAIRE |
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