Functional Analysis of a Bacitracin Resistance Determinant Located on ICE Cp1 , a Novel Tn 916 -Like Element from a Conjugative Plasmid in Clostridium perfringens
| Autor: | Xuxia Yan, Xiaoyan Han, Trudi L. Bannam, Julian I. Rood, Dieter M. Bulach, Xiang-Dang Du, Luke C Southey, Torsten Seemann |
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| Rok vydání: | 2015 |
| Předmět: |
Clostridium perfringens
ATP-binding cassette transporter Bacitracin Biology medicine.disease_cause Enterococcus faecalis Microbiology Plasmid Mechanisms of Resistance medicine Pharmacology (medical) Gene Regulator gene Pharmacology Genetics Structural gene biochemical phenomena metabolism and nutrition biology.organism_classification Anti-Bacterial Agents stomatognathic diseases Infectious Diseases Genes Bacterial Conjugation Genetic Plasmids medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 59:6855-6865 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.01643-15 |
| Popis: | Bacitracins are mixtures of structurally related cyclic polypeptides with antibiotic properties. They act by interfering with the biosynthesis of the bacterial cell wall. In this study, we analyzed an avian necrotic enteritis strain of Clostridium perfringens that was resistant to bacitracin and produced NetB toxin. We identified a bacitracin resistance locus that resembled a bacitracin resistance determinant from Enterococcus faecalis . It contained the structural genes bcrABD and a putative regulatory gene, bcrR . Mutagenesis studies provided evidence that both bcrA and bcrB are essential for bacitracin resistance, and that evidence was supported by the results of experiments in which the introduction of both the bcrA and bcrB genes into a bacitracin-susceptible C. perfringens strain was required to confer bacitracin resistance. The wild-type strain was shown to contain at least three large, putatively conjugative plasmids, and the bcrRABD locus was localized to an 89.7-kb plasmid, pJIR4150. This plasmid was experimentally shown to be conjugative and was sequenced. The sequence revealed that it also carries a tpeL toxin gene and is related to the pCW3 family of conjugative antibiotic resistance and toxin plasmids from C. perfringens . The bcr genes were located on a genetic element, ICE Cp1 , which is related to the Tn 916 family of integrative conjugative elements (ICEs). ICE Cp1 appears to be the first Tn 916 -like element shown to confer bacitracin resistance. In summary, we identified in a toxin-producing C. perfringens strain a novel mobile bacitracin resistance element which was experimentally shown to be essential for bacitracin resistance and is carried by a putative ICE located on a conjugative plasmid. |
| Databáze: | OpenAIRE |
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