Distribuição de células da resposta imune na uretra pélvica e em prepúcio de carneiros

Autor: Jorge Acosta-Dibarrat, Roberto Montes de Oca-Jiménez, Jorge Tórtora-Pérez, Edgardo Soriano-Vargas, Alejandro Buendía-Jiménez
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Pesquisa Veterinária Brasileira, Volume: 34, Issue: 3, Pages: 270-276, Published: MAR 2014
Pesquisa Veterinária Brasileira, Vol 34, Iss 3, Pp 270-276 (2014)
Pesquisa Veterinária Brasileira v.34 n.3 2014
Pesquisa Veterinária Brasileira
Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
Popis: The pathogens of the reproductive system in the male can penetrate and establish by ascending route, from to the prepuce to the urethra, accessory glands, epididymis and testicles. The aim of this paper is determine the distribution and number of cells involved in the immune response in prepuce and pelvic urethra of rams, without apparent clinical alterations in testicle, epididymis and prepuce. The distribution of some of the cells involved in the immune response at the level of the prepuce and the pelvic urethra was quantified in four one-year-old rams seronegative for B. ovis and A. seminis and without apparent lesions in the testicles, the epididymis, and the prepuce. At the moment of slaughter, samples were taken from the preputial fornix and the pelvic urethra and placed in 10% formalin and under freezing conditions. CD4, CD8, WC1, CD45RO, CD14 and CD1b cells were demonstrated by immunohistochemistry, and immunoglobulin-containing cells (ICC) of the IgA, IgG and IgM classes were demonstrated by immunofluorescence. The labeled cells present in the mucosa of both organs were counted with an image analyzer. The total number of cells was compared between both tissues and differentially between the epithelium and the connective tissue of the mucosa. Significant differences were found in the total number of CD4, CD45RO, and WC1 lymphocytes, in CD14 macrophages, and CD1b dendritic cells, with mean values being greater in the fornix than in the urethra (p
Databáze: OpenAIRE