The effect of intracerebroventricular infusions of ghrelin or short fasting on the gene expression and immunoreactivity of neuropeptide Y in the hypothalamic neurons in prepubertal female lambs: A morphofunctional study
| Autor: | Jolanta Polkowska, Marta Wańkowska, Tomasz Misztal, Anna Wójcik-Gładysz, Alina Gajewska |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Time Factors Central nervous system Hypothalamus Biology Random Allocation Cellular and Molecular Neuroscience Basal (phylogenetics) Internal medicine Gene expression medicine Animals Neuropeptide Y Sheep Domestic Neurons Messenger RNA digestive oral and skin physiology Age Factors Fasting Neuropeptide Y receptor Ghrelin humanities Up-Regulation Infusions Intraventricular Treatment Outcome Endocrinology medicine.anatomical_structure Gene Expression Regulation Immunohistochemistry Female hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Chemical Neuroanatomy. 46:45-50 |
| ISSN: | 0891-0618 |
| DOI: | 10.1016/j.jchemneu.2012.10.002 |
| Popis: | The role of exogenous ghrelin in the regulation of neuropeptide Y (NPY) neuronal system in the hypothalamus of intact lambs has not been yet determined. The aim of present study was to investigate the effects of intracerebroventricular infusion of ghrelin or short fasting on the secretory activity of the NPY neurons in the hypothalamus of prepubertal female sheep. Animals (n = 30) were randomly divided into three groups, two groups were fed standard diet and one group was fasted for 72 h. One group fed standard diet and fasted group were infused to the 3rd ventricle of the brain with vehicle, while the remaining group fed standard diet was infused with ghrelin (25 μg/120 μl/h) for 6 h during three consecutive days. Immediately after the treatment, tissues were collected. Parts of the brains were fixed in situ for further immunohistochemical analysis, and remaining parts were frozen for RT-PCR analysis. Both, fasting and ghrelin infusion elicited the same kind of changes in the mRNA and intra-neuronal levels of the NPY hypothalamic neurons. Namely, the expression of NPY mRNA in the medial basal hypothalamus and immunoreactivity of NPY in the arcuate and periventricular nuclei increased in fasted and standard fed with ghrelin's infusion groups compared to standard fed sheep (P < 0.05). These data demonstrate that ghrelin takes part in the mechanisms linking the nutritional status with an activity of the hypothalamic NPY at the level of the central nervous system by stimulating NPY secretion in sheep. |
| Databáze: | OpenAIRE |
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