Using Cryo-EM to Investigate Bacterial Secretion Systems
| Autor: | Chiara Rapisarda, Matteo Tassinari, Rémi Fronzes, Francesca Gubellini |
|---|---|
| Přispěvatelé: | Structure et Fonction des Nanomachines Bactériennes - Structure and function of bacterial nano-machines [IECB, Bordeaux], Institut Européen de Chimie et Biologie (IECB), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Microbiologie Fondamentale et Pathogénicité (MFP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Microbiologie structurale - Structural Microbiology (Microb. Struc. (UMR_3528 / U-Pasteur_5)), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Work in the R.F. lab is supported by the CNRS, ERC, and IdeX. F.G. and M.T. are supported by the Unit of Microbiology directed by Pedro Alzari at the Institut Pasteur. F.G. is funded by the Institut Pasteur. C.R. is funded by IdeX, and M.T. is funded by the Pasteur Paris University (PPU) International PhD Program., We would like to thank Olivera Francetic and Julien Bergeron for critical discussion and comments on the manuscript, and Robin Anger for help with figures. We apologize to all the researchers whose work we did not include in the review because of space constraints., Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), Microbiologie Fondamentale et Pathogénicité [Bordeaux] (MFP), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
MESH: Cryoelectron Microscopy/methods Cryo-electron microscopy Chemistry [SDV]Life Sciences [q-bio] Cryoelectron Microscopy 030106 microbiology cryo-electron microscopy single particle tomography Pathogenicity Microbiology secretion systems MESH: Bacterial Secretion Systems/ultrastructure MESH: Cryoelectron Microscopy/trends 03 medical and health sciences 030104 developmental biology MESH: Bacteria/enzymology Biophysics cryo-EM Secretion bacteria Bacterial Secretion Systems |
| Zdroj: | Annual Review of Microbiology Annual Review of Microbiology, 2018, 72, pp.231-254. ⟨10.1146/annurev-micro-090817-062702⟩ Annual Review of Microbiology, Annual Reviews, 2018, 72, pp.231-254. ⟨10.1146/annurev-micro-090817-062702⟩ |
| ISSN: | 0066-4227 |
| DOI: | 10.1146/annurev-micro-090817-062702⟩ |
| Popis: | International audience; Bacterial secretion systems are responsible for releasing macromolecules to the extracellular milieu or directly into other cells. These membrane complexes are associated with pathogenicity and bacterial fitness. Understanding of these large assemblies has exponentially increased in the last few years thanks to electron microscopy. In fact, a revolution in this field has led to breakthroughs in characterizing the structures of secretion systems and other macromolecular machineries so as to obtain high-resolution images of complexes that could not be crystallized. In this review, we give a brief overview of structural advancements in the understanding of secretion systems, fo-cusing in particular on cryo-electron microscopy, whether tomography or single-particle analysis. We describe how such techniques have contributed to knowledge of the mechanism of macromolecule secretion in bacteria and the impact they will have in the future. |
| Databáze: | OpenAIRE |
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