Palmitic acid induces ceramide accumulation, mitochondrial protein hyperacetylation, and mitochondrial dysfunction in porcine oocytes†
| Autor: | Koumei Shirasuna, Takehito Kuwayama, Nobuhiko Itami, Hisataka Iwata |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Ceramide SIRT3 Swine Palmitic Acid AMP-Activated Protein Kinases Biology Ceramides Mitochondrial Proteins Dephosphorylation 03 medical and health sciences chemistry.chemical_compound Adenosine Triphosphate 0302 clinical medicine Sirtuin 3 Animals Phosphorylation Protein kinase A Cells Cultured chemistry.chemical_classification Reactive oxygen species Cumulus Cells 030219 obstetrics & reproductive medicine Activator (genetics) AMPK Acetylation Cell Biology General Medicine Mitochondria Cell biology Oxidative Stress 030104 developmental biology Reproductive Medicine chemistry Oocytes Female Reactive Oxygen Species |
| Zdroj: | Biology of Reproduction. 98:644-653 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1093/biolre/ioy023 |
| Popis: | Low oocyte quality is a possible causal factor of obesity-induced infertility. High palmitic acid (PA) concentration in follicular fluid is a crucial feature noted in obese women. This study examined how high PA concentration reduced mitochondrial quality in oocytes and investigated a possible countermeasure against mitochondrial dysfunction. Cumulus cell-oocyte complexes were obtained from the ovaries of gilts, and incubated in medium containing PA (0.5 mM) or vehicle (BSA) for 44 h. Culturing oocytes at high PA concentration induced mitochondrial dysfunction determined by high reactive oxygen species and low ATP content in oocytes. Furthermore, high PA levels increased mitochondrial acetylation levels determined by a high degree of co-localization of TOMM20 and acetylated-lysine. In addition, high PA levels reduced the expression of Sirtuin 3 (SIRT3) and phosphorylated AMP-activated protein kinase (AMPK), while the AMPK activator, AICAR, restored mitochondrial function as well as oocyte ability and reduced the acetylation of mitochondrial protein. Supplementation of culture medium with dorsomorphin dihydrochloride (an AMPK inhibitor) reduced mitochondrial function and increased mitochondrial protein acetylation. Treatment of oocytes with LB100 (an inhibitor of AMPK dephosphorylation) reduced mitochondrial acetylation levels and restored mitochondrial function. Furthermore, high PA levels increased ceramide accumulation in oocytes, and addition of ceramide to the culture medium also induced mitochondrial dysfunction and increased mitochondrial acetylation. This detrimental effect of ceramide was diminished by AICAR treatment of oocytes. Our results indicated that PA induces ceramide accumulation and downregulates the AMPK/SIRT3 pathway causing mitochondrial protein hyperacetylation and dysfunction in oocytes. |
| Databáze: | OpenAIRE |
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