Multimodal Characterization of Neural Networks Using Highly Transparent Electrode Arrays
| Autor: | Gergely Katona, George G. Malliaras, Adam Williamson, Attila Kaszás, Mary J. Donahue, Andrea Slézia, Balázs Rózsa, Ivo Vanzetta, Gergely F. Turi, Christophe Bernard |
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| Přispěvatelé: | Département Bioélectronique (BEL-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-CMP-GC, Institut de Neurosciences de la Timone (INT), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Columbia University [New York], New York State Psychiatric Institute, Hungarian Academy of Sciences (MTA), Institut de Neurosciences des Systèmes (INS), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Microbiology [Szeged], University of Szeged [Szeged], Pázmány Péter Catholic University, Fondation pour la Recherche Médicale Grant DBS20131128446KFI-2016-0177GINOP-2016-00979NVKP-2016-0043, European Project: 716867,ERC, European Project: 625372,EC:FP7:PEOPLE,FP7-PEOPLE-2013-IEF,IMAGINE(2015), European Project: 682426,H2020,ERC-2015-CoG,VISONby3DSTIM(2016), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Donahue, Mary J [0000-0002-9158-4026], Kaszas, Attila [0000-0002-2019-3722], Turi, Gergely F [0000-0001-5651-9459], Slézia, Andrea [0000-0002-4528-3169], Bernard, Christophe [0000-0003-3014-1966], Malliaras, George G [0000-0002-4582-8501], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
transparent electronics Materials science [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology [SDV]Life Sciences [q-bio] Population Mice Transgenic Neuroimaging 02 engineering and technology Novel Tools and Methods 03 medical and health sciences Mice PEDOT:PSS Microscopy Biological neural network Animals education Methods/New Tools ComputingMilieux_MISCELLANEOUS 030304 developmental biology Conductive polymer 0303 health sciences education.field_of_study General Neuroscience Brain neuroengineering General Medicine Neural engineering 021001 nanoscience & nanotechnology electrophysiology Electrodes Implanted organic electronics Microelectrode 7.2 Electrode Nerve Net 0210 nano-technology two-photon imaging Biomedical engineering |
| Zdroj: | eNeuro eNeuro, 2019, 5 (6), pp.ENEURO.0187-18.2018. ⟨10.1523/ENEURO.0187-18.2018⟩ eNeuro, Society for Neuroscience, 2019, 5 (6), pp.ENEURO.0187-18.2018. ⟨10.1523/ENEURO.0187-18.2018⟩ eneuro |
| ISSN: | 2373-2822 |
| DOI: | 10.1523/ENEURO.0187-18.2018⟩ |
| Popis: | Visual Abstract Transparent and flexible materials are attractive for a wide range of emerging bioelectronic applications. These include neural interfacing devices for both recording and stimulation, where low electrochemical electrode impedance is valuable. Here the conducting polymer poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) is used to fabricate electrodes that are small enough to allow unencumbered optical access for imaging a large cell population with two-photon (2P) microscopy, yet provide low impedance for simultaneous high quality recordings of neural activity in vivo. To demonstrate this, pathophysiological activity was induced in the mouse cortex using 4-aminopyridine (4AP), and the resulting electrical activity was detected with the PEDOT:PSS-based probe while imaging calcium activity directly below the probe area. The induced calcium activity of the neuronal network as measured by the fluorescence change in the cells correlated well with the electrophysiological recordings from the cortical grid of PEDOT:PSS microelectrodes. Our approach provides a valuable vehicle for complementing classical high temporal resolution electrophysiological analysis with optical imaging. |
| Databáze: | OpenAIRE |
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