Molecular characterization and expression of a disintegrin and metalloproteinase with thrombospondin motifs 8 in chicken
| Autor: | Sung-Jo Kim, Hak-Kyo Lee, Yu Ra Kim, Seokhyun Lee, Ra Ham Lee, Ki-Duk Song |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Protein Structure lcsh:Animal biochemistry Gene Expression ADAMTS8 Biology Article 03 medical and health sciences Gene expression lcsh:QP501-801 Gene lcsh:SF1-1100 Thrombospondin Messenger RNA Molecular biology Chicken Animal Biotechnology 030104 developmental biology Real-time polymerase chain reaction TLR3 Animal Science and Zoology Innate Immune Receptor Signaling lcsh:Animal culture Signal transduction Food Science |
| Zdroj: | Asian-Australasian Journal of Animal Sciences Asian-Australasian Journal of Animal Sciences, Vol 31, Iss 8, Pp 1366-1372 (2018) |
| ISSN: | 1976-5517 1011-2367 |
| Popis: | Objective A disintegrin and metallopeptidase with thrombospondin motifs type 8 (ADAMTS8) is crucial for diverse physiological processes, such as inflammation, tissue morphogenesis, and tumorigenesis. The chicken ADAMTS8 (chADAMTS8) gene was differentially expressed in the kidney following exposure to different calcium concentrations, suggesting a pathological role of this protein in metabolic diseases. We aimed to examine the molecular characteristics of chADAMTS8 and analyze the gene-expression differences in response to toll-like receptor 3 (TLR3) stimulation. METHODS The ADAMTS8 mRNA and amino acid sequences of various species (chicken, duck, cow, mouse, rat, human, chimpanzee, pig, and horse) were retrieved from the Ensembl database and subjected to bioinformatics analyses. Reverse-transcription polymerase chain reaction (RT-PCR) and quantitative PCR (qPCR) experiments were performed with various chicken tissues and the chicken fibroblast DF-1 cell line, which was stimulated with polyinosinic-polycytidylic acid (poly[I:C]; a TLR3 ligand). RESULTS The chADAMTS8 gene was predicted to contain three thrombospondin type 1 (TSP1) domains, whose amino acid sequences shared homology among the different species, whereas sequences outside the TSP1 domains (especially the amino-terminal region) were very difffferent. Phylogenetic analysis revealed that chADAMTS8 is evolutionarily clustered in the same clade with that of the duck. chADAMTS8 mRNA was broadly expressed in chicken tissues, and the expression was significantly up-regulated in the DF-1 cells in response to poly(I:C) stimulation (p |
| Databáze: | OpenAIRE |
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