Strain-Specific Differences in the Expression and Activity of Ogg1 in the CNS
| Autor: | Fernando Cardozo-Pelaez, Juan Sanchez-Ramos, Todd Stedeford, Diana I. Mosquera |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
Cerebellum DNA Repair DNA damage DNA repair Neurotoxins Article Gene Expression Regulation Enzymologic Superoxide dismutase Mice chemistry.chemical_compound Species Specificity Genetics medicine Animals Deoxyguanosine Genetic Predisposition to Disease Molecular Biology Neurons chemistry.chemical_classification Mice Inbred BALB C Mice Inbred ICR biology Strain (chemistry) Superoxide Dismutase Brain Neurodegenerative Diseases Drug Tolerance Molecular biology Mice Inbred C57BL Enzyme medicine.anatomical_structure DNA-Formamidopyrimidine Glycosylase chemistry Biochemistry 8-Hydroxy-2'-Deoxyguanosine biology.protein DNA DNA Damage |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1052-2166 |
| Popis: | The expression and activity of 8-oxoguanosine DNA-glycosylase (Ogg1), a key enzyme responsible for the clearance of the oxidized DNA base 8-hydroxy-2′-deoxyguanosine (oxo8dG), was determined in the cerebellum (CB) and the caudate and the putamen (CP) of male Balb/c, ICR, and C57BL/J mice. There was no significant difference in the protein expression of Ogg1 in the CB or CP. The activity of Ogg1 was not significantly different in the CB; however, in the CP of ICR mice, the activity of Ogg1 was 34% and 31% lower than Balb/c and C57BL/J, respectively. In contrast, the levels of oxo8dG in the CB and CP of C57BL/J mice were nearly twice as high as the values in both regions of Balb/c and ICR mice. The activity of superoxide dismutases (SOD) appeared to account for the differences in the levels of oxo8dG in the C57BL/J strain. Total SOD in the C57BL/J strain was two- and fourfold higher in the CB and CP, respectively, versus the other strains. These results suggest that the enhanced vulnerability of the C57BL/J strain to neurotoxicants may not be due to a decreased capacity for DNA repair, but rather, the significantly higher activity of SODs, which may cause these pathways to become more readily saturated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|