Multiplex three-dimensional brain gene expression mapping in a mouse model of Parkinson's disease
| Autor: | Desmond J. Smith, Arshad H. Khan, William P. Melega, Goran Lacan, Alex Ossadtchi, Simon Yee, Richard M. Leahy, Vanessa M. Brown, Simon R. Cherry |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Parkinson's disease Computational biology Biology computer.software_genre Brain mapping Article Methamphetamine Mice Imaging Three-Dimensional Voxel Gene expression Genetics medicine Image Processing Computer-Assisted Animals natural sciences Parkinson Disease Secondary Gene Genetics (clinical) Regulation of gene expression Brain Chemistry Brain Mapping Gene Expression Profiling Parkinson Disease medicine.disease Gene expression profiling Mice Inbred C57BL Disease Models Animal Gene Expression Regulation Multigene Family Central Nervous System Stimulants DNA microarray computer |
| Zdroj: | Genome research. 12(6) |
| ISSN: | 1088-9051 |
| Popis: | To facilitate high-throughput 3D imaging of brain gene expression, a new method called voxelation has been developed. Spatially registered voxels (cubes) are analyzed, resulting in multiple volumetric maps of gene expression analogous to the images reconstructed in biomedical imaging systems. Using microarrays, 40 voxel images for 9000 genes were acquired from brains of both normal mice and mice in which a pharmacological model of Parkinson's disease (PD) had been induced by methamphetamine. Quality-control analyses established the reproducibility of the voxelation procedure. The investigation revealed a common network of coregulated genes shared between the normal and PD brain, and allowed identification of putative control regions responsible for these networks. In addition, genes involved in cell/cell interactions were found to be prominently regulated in the PD brains. Finally, singular value decomposition (SVD), a mathematical method used to provide parsimonious explanations of complex data sets, identified gene vectors and their corresponding images that distinguished between normal and PD brain structures, most pertinently the striatum.[All study results and supplementary data are available on the web athttp://www.pharmacology.ucla.edu/smithlab/genome_multiplex and athttp://www.genome.org. Microarray data are also available at GEO,http://www.ncbi.nlm.nih.gov/geo, under the series accession no. GSE30.] |
| Databáze: | OpenAIRE |
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