Branch Migration Activity of Rad54 Protein
| Autor: | Alexander V. Mazin, Olga M. Mazina |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Chemistry Hydrolysis fungi 030303 biophysics DNA Helicases DNA Article Branch migration Cell biology DNA-Binding Proteins Meiosis 03 medical and health sciences Adenosine Triphosphate Holliday junction Humans DNA Breaks Double-Stranded Electrophoresis Polyacrylamide Gel Homologous recombination Helicase activity 030304 developmental biology |
| Zdroj: | Homologous Recombination ISBN: 9781071606438 Methods Mol Biol |
| Popis: | Rad54 is a eukaryotic protein that plays an important role in homologous recombination. Rad54, a member of the Swi2/Snf2 family, binds to Holliday junctions with high specificity and promotes their branch migration in an ATP hydrolysis-dependent manner. Here we describe the methods our laboratory used to characterize the branch migration activity of Rad54. These assays are applicable for other branch migration proteins regardless of whether they have canonical helicase activity or not. |
| Databáze: | OpenAIRE |
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