Abnormal placental CD8+ T cell infiltration is a feature of fetal growth restriction and pre-eclampsia
| Autor: | Lisa Happerfield, F. A. Jessop, Ulla Sovio, Cansu Yazar, Elizabeth Eddershaw, Neil J. Sebire, Susanne Lager, Gordon C. S. Smith, D. Stephen Charnock-Jones, Emma Cook, Margaretha W van der Linden |
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| Přispěvatelé: | Lager, Susanne [0000-0003-3556-065X], Sovio, Ulla [0000-0002-0799-1105], Yazar, Cansu [0000-0003-2660-0111], Cook, Emma [0000-0002-6142-4443], Sebire, Neil J [0000-0001-5348-9063], Charnock-Jones, D Stephen [0000-0002-2936-4890], Smith, Gordon CS [0000-0003-2124-0997], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
CD31 Pathology medicine.medical_specialty Physiology leukocytes Placenta Reproduktionsmedicin och gynekologi CD8-Positive T-Lymphocytes 03 medical and health sciences small for gestational age villitis of unknown aetiology 0302 clinical medicine Pre-Eclampsia Pregnancy image analysis Obstetrics Gynecology and Reproductive Medicine medicine Humans small-for-gestational age fibrin Pathological villous tissue Fetal Growth Retardation Eclampsia business.industry Infant Newborn Endothelial Cells Gestational age medicine.disease endothelial cells 030104 developmental biology medicine.anatomical_structure stereology Small for gestational age Female pregnancy complication business pathologist scoring Infiltration (medical) 030217 neurology & neurosurgery |
| Popis: | KEY POINTS: Placental pathological abnormalities are more frequently observed in complicated pregnancies than in healthy pregnancies Infiltration of CD8+ T-cells into the placental villous tissue occurred in both fetal growth restriction and pre-eclampsia, whereas CD79α+ B-cell infiltration was only apparent with reduced fetal growth. Vascularisation, fibrin depositions, macrophage and neutrophil infiltration in the placenta did not differ between healthy and complicated pregnancies. ABSTRACT: Fetal growth restriction (FGR) and pre-eclampsia are severe, adverse pregnancy outcomes. Alterations in placental histology are frequently reported in these pregnancy complications and are often based upon scoring by pathologists. However, many alterations are also observed in placenta from uncomplicated pregnancies. Moreover, knowledge of disease state may bias assessment. We sought to perform an objective comparison of placental microscopic appearance in normal and complicated pregnancies. Placental villous tissue (n = 823) and edge biopsies (n = 488) from 871 individual, singleton pregnancies were collected after delivery. Cases of small-for-gestational-age (SGA) or pre-eclampsia were matched with healthy controls. A subset of the SGA cases displayed signs of FGR. Cases of pre-term delivery were also included. Tissue sections were stained with H&E or antibodies for CD8, CD14, CD31, CD79α, elastase. Images were scored by two experienced pathologists for pathological features or analysed by image analysis and stereology. Analyses were performed blind to case-control status and gestational age. Volume fraction of T-cells increased in placentas from pregnancies complicated by pre-eclampsia (adjusted odds ratio (aOR) 1.46, 95% CI 1.12-1.90) and FGR (aOR 1.64, 95% CI 1.11-2.43), whereas B-cells only increased in FGR (aOR 1.65, 95% CI 1.05-2.60). Pathological abnormalities in villous tissue were reported in 21.4% (88/411) of complicated pregnancies and 14.3% (52/363) of controls (OR 1.62, 95% CI 1.12-2.37). There were no differences in the fractions of endothelial cells, fibrin deposition, macrophages, and neutrophils when comparing normal and complicated pregnancies. In conclusion, FGR and pre-eclampsia are associated with T-cell infiltration of the placenta and placental pathological abnormalities. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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