Antipsychotic clozapine binding to alpha-2-macroglobulin protects interacting partners against oxidation and preserves the anti-proteinase activity of the protein
| Autor: | Nikola Gligorijević, Miloš Šunderić, Olgica Nedić, Miloš Milčić, Čedo Miljević, Milan Nikolić, Tamara Vasović |
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| Rok vydání: | 2021 |
| Předmět: |
Conformational change
Protein Conformation medicine.drug_class Atypical antipsychotic Protein-ligand interaction 02 engineering and technology Biochemistry alpha-2-Macroglobulin Structure-Activity Relationship 03 medical and health sciences Structural Biology medicine Humans alpha-Macroglobulins Binding site Clozapine Molecular Biology Protein secondary structure 030304 developmental biology 0303 health sciences Binding Sites biology Chemistry Hydrogen Bonding General Medicine 021001 nanoscience & nanotechnology Binding constant 3. Good health Molecular Docking Simulation Oxidative Stress Proteostasis biology.protein Biophysics Alpha-2-macroglobulin 0210 nano-technology Hydrophobic and Hydrophilic Interactions Oxidation-Reduction Antipsychotic Agents Protein Binding medicine.drug |
| Zdroj: | International Journal of Biological Macromolecules |
| ISSN: | 0141-8130 |
| Popis: | In this study, the interaction between clozapine, an atypical antipsychotic drug, and alpha-2-macroglobulin (α2M), a multipurpose anti-proteinase, was investigated under simulated (patho) physiological conditions using multiple spectroscopic techniques and molecular modeling. It was found that α2M binds clozapine with a moderate affinity (the binding constant of 0.9 × 105 M−1 at 37 °C). The preferable binding site for both clozapine's atropisomers was revealed to be a large pocket at the interface of C and D monomer subunits of the protein. Hydrogen bonds and the hydrophobic effect were proposed as dominant forces in complex formation. The binding of clozapine did not induce significant conformational change of the protein, as confirmed by virtually unaltered α2M secondary structure and anti-proteinase activity. However, both clozapine and α2M shielded each other from the deleterious influence of strong oxidants: sodium hypochlorite and 2,2′-azobis-2-methyl-propanimidamide dihydrochloride (AAPH). Moreover, clozapine in a concentration range that is usually targeted in the plasma during patients' treatment effectively protected the anti-proteinase activity of α2M under AAPH-induced free radical overproduction. Our results suggest that the cooperation between α2M and clozapine may be a path by which these two molecules synergistically protect neural tissue against injury caused by disturbed proteostasis or oxidative stress. Supplementary material: [https://cherry.chem.bg.ac.rs/handle/123456789/4541] |
| Databáze: | OpenAIRE |
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