Anisotropic engineered heart tissue made from laser-cut decellularized myocardium
| Autor: | Jonas Schwan, Yibing Qyang, Andrea T. Kwaczala, Yongming Ren, Lorenzo R. Sewanan, Oscar Bartulos, Aaron H. Morris, Stuart G. Campbell, Daniel Jacoby, Thomas J. Ryan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Scaffold Materials science Swine Induced Pluripotent Stem Cells Cell Culture Techniques Mechanotransduction Cellular Article 03 medical and health sciences Tissue engineering Animals Myocytes Cardiac Mechanotransduction Anisotropy Induced pluripotent stem cell Polytetrafluoroethylene Cells Cultured Embryonic Stem Cells Multidisciplinary Decellularization Tissue Engineering Tissue Scaffolds Myocardium Anatomy Brain natriuretic peptide Myocardial Contraction Embryonic stem cell Rats 030104 developmental biology Lasers Gas Triiodothyronine Tomography Optical Coherence Biomedical engineering |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep32068 |
| Popis: | We have developed an engineered heart tissue (EHT) system that uses laser-cut sheets of decellularized myocardium as scaffolds. This material enables formation of thin muscle strips whose biomechanical characteristics are easily measured and manipulated. To create EHTs, sections of porcine myocardium were laser-cut into ribbon-like shapes, decellularized and mounted in specialized clips for seeding and culture. Scaffolds were first tested by seeding with neonatal rat ventricular myocytes. EHTs beat synchronously by day five and exhibited robust length-dependent activation by day 21. Fiber orientation within the scaffold affected peak twitch stress, demonstrating its ability to guide cells toward physiologic contractile anisotropy. Scaffold anisotropy also made it possible to probe cellular responses to stretch as a function of fiber angle. Stretch that was aligned with the fiber direction increased expression of brain natriuretic peptide, but off-axis stretches (causing fiber shear) did not. The method also produced robust EHTs from cardiomyocytes derived from human embryonic stem cells and induced pluripotent stem cells (hiPSC). hiPSC-EHTs achieved maximum peak stress of 6.5 mN/mm2 and twitch kinetics approaching reported values from adult human trabeculae. We conclude that laser-cut EHTs are a viable platform for novel mechanotransduction experiments and characterizing the biomechanical function of patient-derived cardiomyoctyes. |
| Databáze: | OpenAIRE |
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